Enhancement of image quality and imaging depth with airy light-sheet microscopy in cleared and non-cleared neural tissue

Jonathan Nylk; Kaley McCluskey; Sanya Aggarwal; Javier A. Tello; Kishan Dholakia

doi:10.1364/BOE.7.004021

Enhancement of image quality and imaging depth with airy light-sheet microscopy in cleared and non-cleared neural tissue

Jonathan Nylk, Kaley McCluskey, Sanya Aggarwal, Javier A. Tello, Kishan Dholakia

Department of Physics

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

We have investigated the effect of Airy illumination on the image quality and depth penetration of digitally scanned light-sheet microscopy in turbid neural tissue. We used Fourier analysis of images acquired using Gaussian and Airy light-sheets to assess their respective image quality versus penetration into the tissue. We observed a three-fold average improvement in image quality at 50 μm depth with the Airy light-sheet. We also used optical clearing to tune the scattering properties of the tissue and found that the improvement when using an Airy light-sheet is greater in the presence of stronger sample-induced aberrations. Finally, we used homogeneous resolution probes in these tissues to quantify absolute depth penetration in cleared samples with each beam type. The Airy light-sheet method extended depth penetration by 30% compared to a Gaussian light-sheet.

Original language	English
Article number	#270350
Pages (from-to)	4021-4033
Number of pages	13
Journal	Biomedical Optics Express
Volume	7
Issue number	10
DOIs	https://doi.org/10.1364/BOE.7.004021
Publication status	Published - 2016 Oct 1

Bibliographical note

Funding Information:
This work was supported by awards to KD from the UK Engineering and Physical Sciences Research Council under grant EP/J01771X/1, the ‘BRAINS’ 600th anniversary appeal, and Dr. E. Killick. We would also like to thank The Northwood Trust and The RS Macdonald Charitable Trust for funding support (awarded to JAT). KD acknowledges support of a Royal Society Leverhulme Trust Senior Fellowship.

Publisher Copyright:
© 2016 Optical Society of America.

All Science Journal Classification (ASJC) codes

Biotechnology
Atomic and Molecular Physics, and Optics

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10.1364/BOE.7.004021

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abstract = "We have investigated the effect of Airy illumination on the image quality and depth penetration of digitally scanned light-sheet microscopy in turbid neural tissue. We used Fourier analysis of images acquired using Gaussian and Airy light-sheets to assess their respective image quality versus penetration into the tissue. We observed a three-fold average improvement in image quality at 50 μm depth with the Airy light-sheet. We also used optical clearing to tune the scattering properties of the tissue and found that the improvement when using an Airy light-sheet is greater in the presence of stronger sample-induced aberrations. Finally, we used homogeneous resolution probes in these tissues to quantify absolute depth penetration in cleared samples with each beam type. The Airy light-sheet method extended depth penetration by 30% compared to a Gaussian light-sheet.",

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AU - Dholakia, Kishan

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Enhancement of image quality and imaging depth with airy light-sheet microscopy in cleared and non-cleared neural tissue

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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