Enhanced surface plasmon microscopy based on multi-channel spatial light switching for label-free neuronal imaging

Taehwang Son; Changhun Lee; Gwiyeong Moon; Dongsu Lee; Eunji Cheong; Donghyun Kim

doi:10.1016/j.bios.2019.111738

Enhanced surface plasmon microscopy based on multi-channel spatial light switching for label-free neuronal imaging

Taehwang Son, Changhun Lee, Gwiyeong Moon, Dongsu Lee, Eunji Cheong, Donghyun Kim

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

In this paper, we have investigated multi-channel switching of light incidence in multiple directions to improve image clarity in surface plasmon microscopy (SPM) for robust and consistent imaging performance regardless of the pattern geometry and shape. Multi-channel light switching in SPM allows significant reduction of adverse scattering effects by surface plasmon (SP). For proof of concept, an eight-channel spatially switched SPM (ssSPM) system has been set up. The results with reference objects including square arrays and Siemens stars experimentally confirm much improved images with ssSPM by reducing the artifacts of SP scattering significantly. On a quantitative basis, contrast analysis preformed with square arrays shows image contrast enhanced by more than three times over conventional SPM. Three image reconstruction algorithms were evaluated for optimal image acquisition. It is suggested that averaging combined with minimum-filtering produces the highest resolution. ssSPM was applied to label-free imaging of primary neuron cultures and shown to present enhanced images with clarity far better than conventional SPM.

Original language	English
Article number	111738
Journal	Biosensors and Bioelectronics
Volume	146
DOIs	https://doi.org/10.1016/j.bios.2019.111738
Publication status	Published - 2019 Dec 15

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation grants funded by the Korean Government ( 2019K2A9A2A08000198 and 2019R1F1A1063602 ). Appendix A

Publisher Copyright:
© 2019 Elsevier B.V.

All Science Journal Classification (ASJC) codes

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/j.bios.2019.111738

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title = "Enhanced surface plasmon microscopy based on multi-channel spatial light switching for label-free neuronal imaging",

abstract = "In this paper, we have investigated multi-channel switching of light incidence in multiple directions to improve image clarity in surface plasmon microscopy (SPM) for robust and consistent imaging performance regardless of the pattern geometry and shape. Multi-channel light switching in SPM allows significant reduction of adverse scattering effects by surface plasmon (SP). For proof of concept, an eight-channel spatially switched SPM (ssSPM) system has been set up. The results with reference objects including square arrays and Siemens stars experimentally confirm much improved images with ssSPM by reducing the artifacts of SP scattering significantly. On a quantitative basis, contrast analysis preformed with square arrays shows image contrast enhanced by more than three times over conventional SPM. Three image reconstruction algorithms were evaluated for optimal image acquisition. It is suggested that averaging combined with minimum-filtering produces the highest resolution. ssSPM was applied to label-free imaging of primary neuron cultures and shown to present enhanced images with clarity far better than conventional SPM.",

author = "Taehwang Son and Changhun Lee and Gwiyeong Moon and Dongsu Lee and Eunji Cheong and Donghyun Kim",

note = "Funding Information: This work was supported by the National Research Foundation grants funded by the Korean Government ( 2019K2A9A2A08000198 and 2019R1F1A1063602 ). Appendix A Publisher Copyright: {\textcopyright} 2019 Elsevier B.V.",

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AU - Lee, Changhun

AU - Moon, Gwiyeong

AU - Lee, Dongsu

AU - Cheong, Eunji

AU - Kim, Donghyun

N1 - Funding Information: This work was supported by the National Research Foundation grants funded by the Korean Government ( 2019K2A9A2A08000198 and 2019R1F1A1063602 ). Appendix A Publisher Copyright: © 2019 Elsevier B.V.

PY - 2019/12/15

Y1 - 2019/12/15

N2 - In this paper, we have investigated multi-channel switching of light incidence in multiple directions to improve image clarity in surface plasmon microscopy (SPM) for robust and consistent imaging performance regardless of the pattern geometry and shape. Multi-channel light switching in SPM allows significant reduction of adverse scattering effects by surface plasmon (SP). For proof of concept, an eight-channel spatially switched SPM (ssSPM) system has been set up. The results with reference objects including square arrays and Siemens stars experimentally confirm much improved images with ssSPM by reducing the artifacts of SP scattering significantly. On a quantitative basis, contrast analysis preformed with square arrays shows image contrast enhanced by more than three times over conventional SPM. Three image reconstruction algorithms were evaluated for optimal image acquisition. It is suggested that averaging combined with minimum-filtering produces the highest resolution. ssSPM was applied to label-free imaging of primary neuron cultures and shown to present enhanced images with clarity far better than conventional SPM.

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Enhanced surface plasmon microscopy based on multi-channel spatial light switching for label-free neuronal imaging

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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