Enhanced operation of femtosecond lasers and applications in cell transfection

Christian T.A. Brown, David J. Stevenson, Xanthi Tsampoula, Craig McDougall, Alexander A. Lagatsky, Wilson Sibbett, Frank J. Gunn-Moore, Kishan Dholakia

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)


In this work we present a review and discussion on the enhancement of femtosecond (fs) lasers for use within biophotonics with a particular focus on their use in optical transfection techniques. We describe the broad range of source options now available for the generation of femtosecond pulses before briefly reviewing the application of fs laser in optical transfection studies. We show that major performance enhancements may be obtained by optimising the spatial and temporal performance of the laser source before considering possible future directions in this field. In relation to optical transfection we describe how such laser sources initiate a multiphoton process to permeate the cell membrane in a transient fashion. We look at aspects of this technique including the ability to combine transfection with optical trapping. For future implementation of such transfection we explore the role of new sources and "nondiffracting" light fields.

Original languageEnglish
Pages (from-to)183-199
Number of pages17
JournalJournal of Biophotonics
Issue number3
Publication statusPublished - 2008

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)


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