Enhanced neurite outgrowth of rat neural cortical cells on surface-modified films of poly(lactic-co-glycolic acid)

Dong Wook Han; Sub Lee Min; Joo Park Bong; Koo Kim Jeong; Jong Chul Park

doi:10.1007/s10529-004-6585-2

Enhanced neurite outgrowth of rat neural cortical cells on surface-modified films of poly(lactic-co-glycolic acid)

Dong Wook Han, Sub Lee Min, Joo Park Bong, Koo Kim Jeong, Jong Chul Park

Department of Medical Engineering

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Neural cortical cells, isolated from prenatal rat cerebra, were grown on surface-modified poly(lactic-coglycolic acid, 65:35) (PLGA) films coated with poly-D-lysine (PDL) with either laminin (LN), fibronectin (FN) or collagen (CN). Immunocytochemistry showed that the isolated cells were highly immunopositive for both neurofilament and MAP-2 with well-organized neurites and somatodendritic localization. The presence of PDL with LN or FN on the PLGA films was essential for increased neural cell growth. Also, PLGA films coated with either PDL/LN or PDL/FN mixtures had higher neurite outgrowth and regular differentiation.

Original language	English
Pages (from-to)	53-58
Number of pages	6
Journal	Biotechnology Letters
Volume	27
Issue number	1
DOIs	https://doi.org/10.1007/s10529-004-6585-2
Publication status	Published - 2005 Jan

Bibliographical note

Funding Information:
This work was supported by Grant No. 02-PJ3-PG3-31402-0018 from the Ministry of Health and Welfare of Korea.

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/s10529-004-6585-2

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title = "Enhanced neurite outgrowth of rat neural cortical cells on surface-modified films of poly(lactic-co-glycolic acid)",

abstract = "Neural cortical cells, isolated from prenatal rat cerebra, were grown on surface-modified poly(lactic-coglycolic acid, 65:35) (PLGA) films coated with poly-D-lysine (PDL) with either laminin (LN), fibronectin (FN) or collagen (CN). Immunocytochemistry showed that the isolated cells were highly immunopositive for both neurofilament and MAP-2 with well-organized neurites and somatodendritic localization. The presence of PDL with LN or FN on the PLGA films was essential for increased neural cell growth. Also, PLGA films coated with either PDL/LN or PDL/FN mixtures had higher neurite outgrowth and regular differentiation.",

author = "Han, {Dong Wook} and Min, {Sub Lee} and Bong, {Joo Park} and Jeong, {Koo Kim} and Park, {Jong Chul}",

note = "Funding Information: This work was supported by Grant No. 02-PJ3-PG3-31402-0018 from the Ministry of Health and Welfare of Korea.",

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AU - Min, Sub Lee

AU - Bong, Joo Park

AU - Jeong, Koo Kim

AU - Park, Jong Chul

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Enhanced neurite outgrowth of rat neural cortical cells on surface-modified films of poly(lactic-co-glycolic acid)

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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