Enhanced cross protection by hetero prime-boost vaccination with recombinant influenza viruses containing chimeric hemagglutinin-M2e epitopes

Bo Ryoung Park; Jeeva Subbiah; Ki Hye Kim; Young Man Kwon; Judy Oh; Min Chul Kim; Chong Hyun Shin; Baik Lin Seong; Sang Moo Kang

doi:10.1016/j.virol.2021.12.003

Enhanced cross protection by hetero prime-boost vaccination with recombinant influenza viruses containing chimeric hemagglutinin-M2e epitopes

Bo Ryoung Park, Jeeva Subbiah, Ki Hye Kim, Young Man Kwon, Judy Oh, Min Chul Kim, Chong Hyun Shin, Baik Lin Seong, Sang Moo Kang

Research output: Contribution to journal › Article › peer-review

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Abstract

Annual repeat influenza vaccination raises concerns about protective efficacy against mismatched viruses. We investigated the impact of heterologous prime-boost vaccination on inducing cross protection by designing recombinant influenza viruses with chimeric hemagglutinin (HA) carrying M2 extracellular domains (M2e-HA). Heterologous prime-boost vaccination of C57BL/6 mice with M2e-HA chimeric virus more effectively induced M2e and HA stalk specific IgG antibodies correlating with cross protection than homologous prime-boost vaccination. Induction of M2e and HA stalk specific IgG antibodies was compromised in 1-year old mice, indicating significant aging effects on priming subdominant M2e and HA stalk IgG antibody responses. This study demonstrates that a heterologous prime-boost strategy with recombinant influenza virus expressing extra M2e epitopes provides more effective cross protection than homologous vaccination.

Original language	English
Pages (from-to)	143-152
Number of pages	10
Journal	Virology
Volume	566
DOIs	https://doi.org/10.1016/j.virol.2021.12.003
Publication status	Published - 2022 Jan

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© 2021 Elsevier Inc.

All Science Journal Classification (ASJC) codes

Virology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virol.2021.12.003

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title = "Enhanced cross protection by hetero prime-boost vaccination with recombinant influenza viruses containing chimeric hemagglutinin-M2e epitopes",

abstract = "Annual repeat influenza vaccination raises concerns about protective efficacy against mismatched viruses. We investigated the impact of heterologous prime-boost vaccination on inducing cross protection by designing recombinant influenza viruses with chimeric hemagglutinin (HA) carrying M2 extracellular domains (M2e-HA). Heterologous prime-boost vaccination of C57BL/6 mice with M2e-HA chimeric virus more effectively induced M2e and HA stalk specific IgG antibodies correlating with cross protection than homologous prime-boost vaccination. Induction of M2e and HA stalk specific IgG antibodies was compromised in 1-year old mice, indicating significant aging effects on priming subdominant M2e and HA stalk IgG antibody responses. This study demonstrates that a heterologous prime-boost strategy with recombinant influenza virus expressing extra M2e epitopes provides more effective cross protection than homologous vaccination.",

author = "Park, {Bo Ryoung} and Jeeva Subbiah and Kim, {Ki Hye} and Kwon, {Young Man} and Judy Oh and Kim, {Min Chul} and Shin, {Chong Hyun} and Seong, {Baik Lin} and Kang, {Sang Moo}",

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year = "2022",

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doi = "10.1016/j.virol.2021.12.003",

language = "English",

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pages = "143--152",

journal = "Virology",

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T1 - Enhanced cross protection by hetero prime-boost vaccination with recombinant influenza viruses containing chimeric hemagglutinin-M2e epitopes

AU - Park, Bo Ryoung

AU - Subbiah, Jeeva

AU - Kim, Ki Hye

AU - Kwon, Young Man

AU - Oh, Judy

AU - Kim, Min Chul

AU - Shin, Chong Hyun

AU - Seong, Baik Lin

AU - Kang, Sang Moo

PY - 2022/1

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N2 - Annual repeat influenza vaccination raises concerns about protective efficacy against mismatched viruses. We investigated the impact of heterologous prime-boost vaccination on inducing cross protection by designing recombinant influenza viruses with chimeric hemagglutinin (HA) carrying M2 extracellular domains (M2e-HA). Heterologous prime-boost vaccination of C57BL/6 mice with M2e-HA chimeric virus more effectively induced M2e and HA stalk specific IgG antibodies correlating with cross protection than homologous prime-boost vaccination. Induction of M2e and HA stalk specific IgG antibodies was compromised in 1-year old mice, indicating significant aging effects on priming subdominant M2e and HA stalk IgG antibody responses. This study demonstrates that a heterologous prime-boost strategy with recombinant influenza virus expressing extra M2e epitopes provides more effective cross protection than homologous vaccination.

AB - Annual repeat influenza vaccination raises concerns about protective efficacy against mismatched viruses. We investigated the impact of heterologous prime-boost vaccination on inducing cross protection by designing recombinant influenza viruses with chimeric hemagglutinin (HA) carrying M2 extracellular domains (M2e-HA). Heterologous prime-boost vaccination of C57BL/6 mice with M2e-HA chimeric virus more effectively induced M2e and HA stalk specific IgG antibodies correlating with cross protection than homologous prime-boost vaccination. Induction of M2e and HA stalk specific IgG antibodies was compromised in 1-year old mice, indicating significant aging effects on priming subdominant M2e and HA stalk IgG antibody responses. This study demonstrates that a heterologous prime-boost strategy with recombinant influenza virus expressing extra M2e epitopes provides more effective cross protection than homologous vaccination.

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Enhanced cross protection by hetero prime-boost vaccination with recombinant influenza viruses containing chimeric hemagglutinin-M2e epitopes

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

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