Enhanced biohydrogen production from beverage industrial wastewater using external nitrogen sources and bioaugmentation with facultative anaerobic strains

Gopalakrishnan Kumar; Péter Bakonyi; Periyasamy Sivagurunathan; Sang Hyoun Kim; Nándor Nemestóthy; Katalin Bélafi-Bakó; Chiu Yue Lin

doi:10.1016/j.jbiosc.2014.12.011

Enhanced biohydrogen production from beverage industrial wastewater using external nitrogen sources and bioaugmentation with facultative anaerobic strains

Gopalakrishnan Kumar, Péter Bakonyi, Periyasamy Sivagurunathan, Sang Hyoun Kim, Nándor Nemestóthy, Katalin Bélafi-Bakó, Chiu Yue Lin

Department of Civil and Environmental Engineering

Research output: Contribution to journal › Article › peer-review

60 Citations (Scopus)

Abstract

In this work biohydrogen generation and its improvement possibilities from beverage industrial wastewater were sought. Firstly, mesophilic hydrogen fermentations were conducted in batch vials by applying heat-treated (80°C, 30 min) sludge and liquid (LB-grown) cultures of Escherichia coli XL1-Blue/. Enterobacter cloacae DSM 16657 strains for bioaugmentation purposes. The results showed that there was a remarkable increase in hydrogen production capacities when facultative anaerobes were added in the form of inoculum. Furthermore, experiments were carried out in order to reveal whether the increment occurred either due to the efficient contribution of the facultative anaerobic microorganisms or the culture ingredients (in particular yeast extract and tryptone) supplied when the bacterial suspensions (LB media-based inocula) were mixed with the sludge. The outcome of these tests was that both the applied nitrogen sources and the bacteria (. E. coli) could individually enhance hydrogen formation. Nevertheless, the highest increase took place when they were used together. Finally, the optimal initial wastewater concentration was determined as 5 g/L.

Original language	English
Pages (from-to)	155-160
Number of pages	6
Journal	Journal of Bioscience and Bioengineering
Volume	120
Issue number	2
DOIs	https://doi.org/10.1016/j.jbiosc.2014.12.011
Publication status	Published - 2015 Aug 1

Bibliographical note

Funding Information:
This work was partially supported by the European Union and financed by the European Social Fund in the frame of the TAMOP-4.2.2/A-11/1/KONV-2012-0071 project and the János Bolyai Research Scholarship of the Hungarian Academy of Sciences . The authors also gratefully acknowledge the financial supports by Taiwan's Bureau of Energy (grant no. 102-D0616 ) and Taiwan's Ministry of Science and Technology ( NSC-102-2221-E-035-002-MY3 and NSC-102-2622E-035-016-CC1 ).

Publisher Copyright:
© 2014 The Society for Biotechnology, Japan.

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jbiosc.2014.12.011

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Kumar, G., Bakonyi, P., Sivagurunathan, P., Kim, S. H., Nemestóthy, N., Bélafi-Bakó, K., & Lin, C. Y. (2015). Enhanced biohydrogen production from beverage industrial wastewater using external nitrogen sources and bioaugmentation with facultative anaerobic strains. Journal of Bioscience and Bioengineering, 120(2), 155-160. https://doi.org/10.1016/j.jbiosc.2014.12.011

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title = "Enhanced biohydrogen production from beverage industrial wastewater using external nitrogen sources and bioaugmentation with facultative anaerobic strains",

abstract = "In this work biohydrogen generation and its improvement possibilities from beverage industrial wastewater were sought. Firstly, mesophilic hydrogen fermentations were conducted in batch vials by applying heat-treated (80°C, 30 min) sludge and liquid (LB-grown) cultures of Escherichia coli XL1-Blue/. Enterobacter cloacae DSM 16657 strains for bioaugmentation purposes. The results showed that there was a remarkable increase in hydrogen production capacities when facultative anaerobes were added in the form of inoculum. Furthermore, experiments were carried out in order to reveal whether the increment occurred either due to the efficient contribution of the facultative anaerobic microorganisms or the culture ingredients (in particular yeast extract and tryptone) supplied when the bacterial suspensions (LB media-based inocula) were mixed with the sludge. The outcome of these tests was that both the applied nitrogen sources and the bacteria (. E. coli) could individually enhance hydrogen formation. Nevertheless, the highest increase took place when they were used together. Finally, the optimal initial wastewater concentration was determined as 5 g/L.",

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note = "Funding Information: This work was partially supported by the European Union and financed by the European Social Fund in the frame of the TAMOP-4.2.2/A-11/1/KONV-2012-0071 project and the J{\'a}nos Bolyai Research Scholarship of the Hungarian Academy of Sciences . The authors also gratefully acknowledge the financial supports by Taiwan's Bureau of Energy (grant no. 102-D0616 ) and Taiwan's Ministry of Science and Technology ( NSC-102-2221-E-035-002-MY3 and NSC-102-2622E-035-016-CC1 ). Publisher Copyright: {\textcopyright} 2014 The Society for Biotechnology, Japan.",

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Kumar, G, Bakonyi, P, Sivagurunathan, P, Kim, SH, Nemestóthy, N, Bélafi-Bakó, K & Lin, CY 2015, 'Enhanced biohydrogen production from beverage industrial wastewater using external nitrogen sources and bioaugmentation with facultative anaerobic strains', Journal of Bioscience and Bioengineering, vol. 120, no. 2, pp. 155-160. https://doi.org/10.1016/j.jbiosc.2014.12.011

Enhanced biohydrogen production from beverage industrial wastewater using external nitrogen sources and bioaugmentation with facultative anaerobic strains. / Kumar, Gopalakrishnan; Bakonyi, Péter; Sivagurunathan, Periyasamy et al.
In: Journal of Bioscience and Bioengineering, Vol. 120, No. 2, 01.08.2015, p. 155-160.

Research output: Contribution to journal › Article › peer-review

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AU - Kumar, Gopalakrishnan

AU - Bakonyi, Péter

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AU - Nemestóthy, Nándor

AU - Bélafi-Bakó, Katalin

AU - Lin, Chiu Yue

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PY - 2015/8/1

Y1 - 2015/8/1

N2 - In this work biohydrogen generation and its improvement possibilities from beverage industrial wastewater were sought. Firstly, mesophilic hydrogen fermentations were conducted in batch vials by applying heat-treated (80°C, 30 min) sludge and liquid (LB-grown) cultures of Escherichia coli XL1-Blue/. Enterobacter cloacae DSM 16657 strains for bioaugmentation purposes. The results showed that there was a remarkable increase in hydrogen production capacities when facultative anaerobes were added in the form of inoculum. Furthermore, experiments were carried out in order to reveal whether the increment occurred either due to the efficient contribution of the facultative anaerobic microorganisms or the culture ingredients (in particular yeast extract and tryptone) supplied when the bacterial suspensions (LB media-based inocula) were mixed with the sludge. The outcome of these tests was that both the applied nitrogen sources and the bacteria (. E. coli) could individually enhance hydrogen formation. Nevertheless, the highest increase took place when they were used together. Finally, the optimal initial wastewater concentration was determined as 5 g/L.

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Enhanced biohydrogen production from beverage industrial wastewater using external nitrogen sources and bioaugmentation with facultative anaerobic strains

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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