Endogenous somatostatin receptors mobilize calcium from inositol 1,4,5-trisphosphate-sensitive stores in NG108-15 cells

Duck Joo Rhie, Jong Ho Sung, U. Sin Ha, Hee Jung Kim, Do Sik Min, Sang June Hahn, Myung Suk Kim, Yang Hyeok Jo, Shin Hee Yoon

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14 Citations (Scopus)


Somatostatin receptors are members of the G-protein-coupled receptor superfamily and exert their principal effects by coupling to inhibitory G-proteins. We used fura-2-based digital calcium imaging and assayed for [3H]inositol phosphates (IPs) to study the effects of somatostatin on intracellular calcium signaling in neuroblastomaXglioma NG108-15 cells. Both somatostatin-14 and octreotide induced concentration-dependent increases in intracellular Ca2+ concentration ([Ca2+]i). Thirty-four percent of the cells responded to treatment with 100 nM somatostatin-14. Somatostatin-induced responses were not blocked by the removal of extracellular calcium; instead, they were abolished by pretreatment with 100 nM thapsigargin, an agent that depletes and prevents refilling of intracellular Ca2+ stores. Pretreatment with the inositol 1,4,5-trisphosphate (IP3) receptor antagonist xestospongin C (10 μM) for 20 min inhibited markedly the somatostatin-induced response. Somatostatin (100 nM) increased [3H]IPs formation. U73122 (1 μM), an inhibitor of phospholipase C (PLC), completely blocked the somatostatin-induced [Ca2+]i increases and the formation of [3H]IPs. Pretreatment with pertussis toxin (PTX, 200 ng/ml) for 24 h blocked the somatostatin-induced responses. Thus, we conclude that activation of endogenous somatostatin receptors in NG108-15 cells induces the release of calcium from IP3-sensitive intracellular stores through PTX-sensitive G-protein-coupled PLC.

Original languageEnglish
Pages (from-to)120-128
Number of pages9
JournalBrain Research
Issue number1-2
Publication statusPublished - 2003 Jun 13

Bibliographical note

Funding Information:
This work was supported by a Korea Research Foundation Grant (KRF-2000-015-FP0021).

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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