Abstract
A highly efficient droplet-generating microfluidic chip for encapsulating cells with hydrogel is presented. The chip has channels of several tens of micrometers for extruding alginate aqueous solution containing the P19 embryonic carcinoma stem cells and immiscible mineral oil. The cross junction of two heterogeneous flows was able to generate alginate droplets based on Rayleigh-Plateau instability. The electrostatic stabilized alginate solution was prepared by mixing 0.05 % poly-L-lysine and 1 % alginate solution. The diameter and the frequency of alginate droplets were controlled by the concentration of alginate solution and the velocity of running fluids. The alginate droplets in oil flowed into reservoir of calcium ion solution and reacted with ions instantaneously to form hydrogel bead. As a result, cells were quickly encapsulated in synthesized alginate hydrogel beads of 100∼200 μm in diameter in microfluidic chip. These encapsulation beads were applicable to the encapsulation of single or several stem cells leading to the embryonic body formation for stem cell differentiation.
| Original language | English |
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| Pages | 1471-1473 |
| Number of pages | 3 |
| Publication status | Published - 2006 |
| Event | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: 2006 Nov 5 → 2006 Nov 9 |
Other
| Other | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 |
|---|---|
| Country/Territory | Japan |
| City | Tokyo |
| Period | 06/11/5 → 06/11/9 |
All Science Journal Classification (ASJC) codes
- Chemical Engineering (miscellaneous)
- Bioengineering