Replication protein A (RPA) is a eukaryotic single-stranded DNA binding protein that is essential for DNA replication, repair, and recombination, and human RPA interacting protein α (hRIPα) is the nuclear transporter of RPA. Here, we report the regulatory role of hRIPα protein in cell proliferation. Western blot analysis revealed that the level of hRIPα was frequently elevated in cervical tumors tissues and hRIPα knockdown by siRNA inhibited cellular proliferation through deregulation of the cell cycle. In addition, overexpression of hRIPα resulted in increased clonogenicity. These results indicate that hRIPα is involved in cell proliferation through regulation of RPA transport.
Bibliographical noteFunding Information:
We are especially grateful to B. Stillman for providing anti-RPA antibody. This study was supported by a National Research Foundation of Korea Grant funded by the Korean Government ( 2010-0009567 ) and by a Grant from the National R&D Program for Cancer Control, Ministry for Health and Welfare, Republic of Korea (1120280).
All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology
- Cell Biology