Elevated interleukin-32 expression in granulomatosis with polyangiitis

Suyoung Bae, Yong Gil Kim, Jida Choi, Jaewoo Hong, Siyoung Lee, Taebong Kang, Hyunjhung Jeon, Kwangwon Hong, Eusom Kim, Areum Kwak, Chang Keun Lee, Bin Yoo, Yong Beom Park, Eun Young Song, Soohyun Kim

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18 Citations (Scopus)


Objective: To evaluate the role of IL-32 in granulomatosis with polyangiitis (GPA) patients and the relationship between IL-32 and disease activity, as PR3 has the ability to bind and activate IL-32, which has been described as a novel cytokine that induces inflammatory cytokines. Methods: We investigated the level of IL-32, PR3, TNF-α and IL-6 in GPA patients by using ELISA. Northern blot was used to analyse the level of IL-32 mRNA in leucocytes of GPA patients. The intracellular colocalization of IL-32 and PR3 in leucocytes was examined by IF staining. Results: We observed that IL-32 and PR3 levels in GPA patients were increased significantly when compared with normal individuals and each was tightly associated (P < 0.001). Northern blot analysis revealed that the mRNA level of IL-32 was prominently elevated in leucocytes of GPA patients. The intracellular colocalization of IL-32 and PR3 in leucocytes from GPA patients vs normal individuals was verified by IF staining. Conclusion: IL-32 level was elevated in GPA patients but its level was changed by treatment response. IL-32 could be an index in GPA and play a role in the aetiology of GPA.

Original languageEnglish
Article numberkes163
Pages (from-to)1979-1988
Number of pages10
JournalRheumatology (United Kingdom)
Issue number11
Publication statusPublished - 2012 Nov

Bibliographical note

Funding Information:
S.B., J.C. and J.H. were supported by a Korea Science and Engineering Foundation (KOSEF) grant funded by the Korean government (WCU: R33-2008-000-10022-0 and KRF-2008-313-C00644).

Funding Information:
Funding: This study was supported by a grant from the Korea Healthcare Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (A100460).

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Pharmacology (medical)


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