Electrospun nanofibrous scaffolds for controlled release of adeno-associated viral vectors

Slgirim Lee; Jung Suk Kim; Hun Su Chu; Go Woon Kim; Jong In Won; Jae Hyung Jang

doi:10.1016/j.actbio.2011.06.035

Electrospun nanofibrous scaffolds for controlled release of adeno-associated viral vectors

Slgirim Lee, Jung Suk Kim, Hun Su Chu, Go Woon Kim, Jong In Won, Jae Hyung Jang

Department of Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

The integration of viral gene delivery with key features of biomaterial scaffolds that modulate viral delivery in a controlled manner offers a promising strategy for numerous tissue engineering applications. In this study adeno-associated virus (AAV), which is widely utilized in human gene therapy as a gene carrier due to its safety and efficient gene delivery capability, was encapsulated within electrospun nanofibrous scaffolds composed of blended mixtures of elastin-like polypeptides (ELP) and poly (ε-caprolactone) (PCL) and was employed to transduce fibroblasts adherent on the scaffolds. Combinatorial interactions between ELP and PCL chains upon physical blending significantly altered the mechanical properties (i.e. wettability, elastic modulus, strain, etc.) of the ELP/PCL composites, thus providing key tools to mediate controlled release of AAV vectors and robust cellular transduction on the fibrous scaffolds. The ability of ELP/PCL composites to manipulate the controlled release of AAV-mediated gene delivery for subsequent high-efficiency cellular transduction will provide tremendous opportunities for a variety of tissue engineering applications.

Original language	English
Pages (from-to)	3868-3876
Number of pages	9
Journal	Acta Biomaterialia
Volume	7
Issue number	11
DOIs	https://doi.org/10.1016/j.actbio.2011.06.035
Publication status	Published - 2011 Nov

Bibliographical note

Funding Information:
This work was supported by a National Research Foundation grant funded by the Korean Government through the Active Polymer Center for Pattern Integration (No. R11-2007-050-00000-0 ), the National Research Foundation ( NRF-20100008547 ), and the Seoul R&BD Program ( 10816 ). Additionally, this work was supported by a Human Resources Development of the Korea Institute of Energy Technology Evaluation and Planning grant funded by the Korean Government Ministry of Knowledge Economy (No. 20104010100500 ). Appendix A

All Science Journal Classification (ASJC) codes

Biotechnology
Biomaterials
Biochemistry
Biomedical Engineering
Molecular Biology

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10.1016/j.actbio.2011.06.035

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abstract = "The integration of viral gene delivery with key features of biomaterial scaffolds that modulate viral delivery in a controlled manner offers a promising strategy for numerous tissue engineering applications. In this study adeno-associated virus (AAV), which is widely utilized in human gene therapy as a gene carrier due to its safety and efficient gene delivery capability, was encapsulated within electrospun nanofibrous scaffolds composed of blended mixtures of elastin-like polypeptides (ELP) and poly (ε-caprolactone) (PCL) and was employed to transduce fibroblasts adherent on the scaffolds. Combinatorial interactions between ELP and PCL chains upon physical blending significantly altered the mechanical properties (i.e. wettability, elastic modulus, strain, etc.) of the ELP/PCL composites, thus providing key tools to mediate controlled release of AAV vectors and robust cellular transduction on the fibrous scaffolds. The ability of ELP/PCL composites to manipulate the controlled release of AAV-mediated gene delivery for subsequent high-efficiency cellular transduction will provide tremendous opportunities for a variety of tissue engineering applications.",

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AU - Won, Jong In

AU - Jang, Jae Hyung

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N2 - The integration of viral gene delivery with key features of biomaterial scaffolds that modulate viral delivery in a controlled manner offers a promising strategy for numerous tissue engineering applications. In this study adeno-associated virus (AAV), which is widely utilized in human gene therapy as a gene carrier due to its safety and efficient gene delivery capability, was encapsulated within electrospun nanofibrous scaffolds composed of blended mixtures of elastin-like polypeptides (ELP) and poly (ε-caprolactone) (PCL) and was employed to transduce fibroblasts adherent on the scaffolds. Combinatorial interactions between ELP and PCL chains upon physical blending significantly altered the mechanical properties (i.e. wettability, elastic modulus, strain, etc.) of the ELP/PCL composites, thus providing key tools to mediate controlled release of AAV vectors and robust cellular transduction on the fibrous scaffolds. The ability of ELP/PCL composites to manipulate the controlled release of AAV-mediated gene delivery for subsequent high-efficiency cellular transduction will provide tremendous opportunities for a variety of tissue engineering applications.

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Electrospun nanofibrous scaffolds for controlled release of adeno-associated viral vectors

Abstract

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