Electrogenerated chemiluminescence ethanol biosensor based on carbon nanotube-titania-Nafion composite film

Nim Choi Han; Hyun Yoon Sook; Young Ku Lyu; Won Yong Lee

doi:10.1002/elan.200603747

Electrogenerated chemiluminescence ethanol biosensor based on carbon nanotube-titania-Nafion composite film

Nim Choi Han, Hyun Yoon Sook, Young Ku Lyu, Won Yong Lee

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Mesoporous titania-Nafion composite doped with carbon nanotube (CNT) has been used for the immobilization of tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)₃²⁺) and alcohol dehydrogenase on an electrode surface to yield a highly sensitive and stable electrogenerated chemiluminescence (ECL) ethanol biosensor. The presence of CNT in the composite film increases not only the sensitivity of the ECL biosensor but also the long-term stability of the biosensor. The present biosensor responds linearly to ethanol in the wide concentration ranges from 1.0 × 10^-5 M to 1.0 × 10^-1 M with a detection limit of 5.0 × 10 ^-6 M (S/N = 3). The present ECL ethanol biosensor exhibited higher ECL response compared to that obtained with the ECL biosensor based on the corresponding composite without CNT. The present CNT-based ECL biosensor showed good long-term stability with 75% of its initial activity retained after 2 weeks of storage in 50 mM phosphate buffer at pH 7.0.

Original language	English
Pages (from-to)	459-465
Number of pages	7
Journal	Electroanalysis
Volume	19
Issue number	4
DOIs	https://doi.org/10.1002/elan.200603747
Publication status	Published - 2007 Feb

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Electrochemistry

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title = "Electrogenerated chemiluminescence ethanol biosensor based on carbon nanotube-titania-Nafion composite film",

abstract = "Mesoporous titania-Nafion composite doped with carbon nanotube (CNT) has been used for the immobilization of tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)32+) and alcohol dehydrogenase on an electrode surface to yield a highly sensitive and stable electrogenerated chemiluminescence (ECL) ethanol biosensor. The presence of CNT in the composite film increases not only the sensitivity of the ECL biosensor but also the long-term stability of the biosensor. The present biosensor responds linearly to ethanol in the wide concentration ranges from 1.0 × 10-5 M to 1.0 × 10-1 M with a detection limit of 5.0 × 10 -6 M (S/N = 3). The present ECL ethanol biosensor exhibited higher ECL response compared to that obtained with the ECL biosensor based on the corresponding composite without CNT. The present CNT-based ECL biosensor showed good long-term stability with 75% of its initial activity retained after 2 weeks of storage in 50 mM phosphate buffer at pH 7.0.",

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AU - Han, Nim Choi

AU - Sook, Hyun Yoon

AU - Lyu, Young Ku

AU - Lee, Won Yong

PY - 2007/2

Y1 - 2007/2

N2 - Mesoporous titania-Nafion composite doped with carbon nanotube (CNT) has been used for the immobilization of tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)32+) and alcohol dehydrogenase on an electrode surface to yield a highly sensitive and stable electrogenerated chemiluminescence (ECL) ethanol biosensor. The presence of CNT in the composite film increases not only the sensitivity of the ECL biosensor but also the long-term stability of the biosensor. The present biosensor responds linearly to ethanol in the wide concentration ranges from 1.0 × 10-5 M to 1.0 × 10-1 M with a detection limit of 5.0 × 10 -6 M (S/N = 3). The present ECL ethanol biosensor exhibited higher ECL response compared to that obtained with the ECL biosensor based on the corresponding composite without CNT. The present CNT-based ECL biosensor showed good long-term stability with 75% of its initial activity retained after 2 weeks of storage in 50 mM phosphate buffer at pH 7.0.

AB - Mesoporous titania-Nafion composite doped with carbon nanotube (CNT) has been used for the immobilization of tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)32+) and alcohol dehydrogenase on an electrode surface to yield a highly sensitive and stable electrogenerated chemiluminescence (ECL) ethanol biosensor. The presence of CNT in the composite film increases not only the sensitivity of the ECL biosensor but also the long-term stability of the biosensor. The present biosensor responds linearly to ethanol in the wide concentration ranges from 1.0 × 10-5 M to 1.0 × 10-1 M with a detection limit of 5.0 × 10 -6 M (S/N = 3). The present ECL ethanol biosensor exhibited higher ECL response compared to that obtained with the ECL biosensor based on the corresponding composite without CNT. The present CNT-based ECL biosensor showed good long-term stability with 75% of its initial activity retained after 2 weeks of storage in 50 mM phosphate buffer at pH 7.0.

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JO - Electroanalysis

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Electrogenerated chemiluminescence ethanol biosensor based on carbon nanotube-titania-Nafion composite film

Abstract

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