Electrochemical ELISA using diamond-like carbon (DLC) microelectrode for RA diagnosis

Jo Il Kim; Jae Chul Pyun

doi:10.1016/j.proche.2009.07.261

Electrochemical ELISA using diamond-like carbon (DLC) microelectrode for RA diagnosis

Jo Il Kim, Jae Chul Pyun

Department of Materials Science and Engineering

Research output: Contribution to journal › Conference article › peer-review

1 Citation (Scopus)

Abstract

A diamond-like carbon (DLC) microelectrode was applied for diagnosis of RA (rheumatoid arthritis). In this work, oxidized 3,5,3',5'-tetramethylbenzidine (TMB) was quantified by amperometry instead of optical density measurement. Quantification was carried out by using a boron doped DLC microelectrode and cyclic voltammetric analysis method without quenching step which uses sulfuric acid. For the RA diagnosis, the cut-off value was determined by considering the sensitivity as well as the selectivity of the analysis. The test results demonstrate that electrochemical ELISA using DLC microelectrode was feasible for the RA diagnosis.

Original language	English
Pages (from-to)	1047-1050
Number of pages	4
Journal	Procedia Chemistry
Volume	1
Issue number	1
DOIs	https://doi.org/10.1016/j.proche.2009.07.261
Publication status	Published - 2009 Sept
Event	Eurosensors 23rd Conference - Lausanne, Switzerland Duration: 2009 Sept 6 → 2009 Sept 9

Bibliographical note

Funding Information:
This work was supported by KOSEF through National Core Research Center for Nanomedical Technology (No. R15-2004-024-00000-0), by the Korea Science and Engineering Foundation (KOSEF) grant founded by the Korea Goverment (MOST) (No. R01-2008-000-11767-0) and (No. 2009-0073809), and by the KIST institutional program.

All Science Journal Classification (ASJC) codes

Chemistry(all)
Chemical Engineering(all)

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10.1016/j.proche.2009.07.261

Cite this

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title = "Electrochemical ELISA using diamond-like carbon (DLC) microelectrode for RA diagnosis",

abstract = "A diamond-like carbon (DLC) microelectrode was applied for diagnosis of RA (rheumatoid arthritis). In this work, oxidized 3,5,3',5'-tetramethylbenzidine (TMB) was quantified by amperometry instead of optical density measurement. Quantification was carried out by using a boron doped DLC microelectrode and cyclic voltammetric analysis method without quenching step which uses sulfuric acid. For the RA diagnosis, the cut-off value was determined by considering the sensitivity as well as the selectivity of the analysis. The test results demonstrate that electrochemical ELISA using DLC microelectrode was feasible for the RA diagnosis.",

author = "Kim, {Jo Il} and Pyun, {Jae Chul}",

note = "Funding Information: This work was supported by KOSEF through National Core Research Center for Nanomedical Technology (No. R15-2004-024-00000-0), by the Korea Science and Engineering Foundation (KOSEF) grant founded by the Korea Goverment (MOST) (No. R01-2008-000-11767-0) and (No. 2009-0073809), and by the KIST institutional program.; Eurosensors 23rd Conference ; Conference date: 06-09-2009 Through 09-09-2009",

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AU - Pyun, Jae Chul

N1 - Funding Information: This work was supported by KOSEF through National Core Research Center for Nanomedical Technology (No. R15-2004-024-00000-0), by the Korea Science and Engineering Foundation (KOSEF) grant founded by the Korea Goverment (MOST) (No. R01-2008-000-11767-0) and (No. 2009-0073809), and by the KIST institutional program.

PY - 2009/9

Y1 - 2009/9

N2 - A diamond-like carbon (DLC) microelectrode was applied for diagnosis of RA (rheumatoid arthritis). In this work, oxidized 3,5,3',5'-tetramethylbenzidine (TMB) was quantified by amperometry instead of optical density measurement. Quantification was carried out by using a boron doped DLC microelectrode and cyclic voltammetric analysis method without quenching step which uses sulfuric acid. For the RA diagnosis, the cut-off value was determined by considering the sensitivity as well as the selectivity of the analysis. The test results demonstrate that electrochemical ELISA using DLC microelectrode was feasible for the RA diagnosis.

AB - A diamond-like carbon (DLC) microelectrode was applied for diagnosis of RA (rheumatoid arthritis). In this work, oxidized 3,5,3',5'-tetramethylbenzidine (TMB) was quantified by amperometry instead of optical density measurement. Quantification was carried out by using a boron doped DLC microelectrode and cyclic voltammetric analysis method without quenching step which uses sulfuric acid. For the RA diagnosis, the cut-off value was determined by considering the sensitivity as well as the selectivity of the analysis. The test results demonstrate that electrochemical ELISA using DLC microelectrode was feasible for the RA diagnosis.

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Electrochemical ELISA using diamond-like carbon (DLC) microelectrode for RA diagnosis

Abstract

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