Electrical impedance monitoring of C2C12 myoblast differentiation on an indium tin oxide electrode

Ilhwan Park; Yeonhee Hong; Young Hoo Jun; Ga Yeon Lee; Hee Sook Jun; Jae Chul Pyun; Jeong Woo Choi; Sungbo Cho

doi:10.3390/s16122068

Electrical impedance monitoring of C2C12 myoblast differentiation on an indium tin oxide electrode

Ilhwan Park, Yeonhee Hong, Young Hoo Jun, Ga Yeon Lee, Hee Sook Jun, Jae Chul Pyun, Jeong Woo Choi, Sungbo Cho

Department of Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Electrical cell-substrate impedance sensing is increasingly being used for label-free and real-time monitoring of changes in cell morphology and number during cell growth, drug screening, and differentiation. In this study, we evaluated the feasibility of using ECIS to monitor C2C12 myoblast differentiation using a fabricated indium tin oxide (ITO) electrode-based chip. C2C12 myoblast differentiation on the ITO electrode was validated based on decreases in the mRNA level of MyoD and increases in the mRNA levels of myogenin and myosin heavy chain (MHC). Additionally, MHC expression and morphological changes in myoblasts differentiated on the ITO electrode were comparable to those in cells in the control culture dish. From the monitoring the integration of the resistance change at 21.5 kHz, the cell differentiation was label-free and real-time detectable in 30 h of differentiation (p < 0.05).

Original language	English
Article number	2068
Journal	Sensors (Switzerland)
Volume	16
Issue number	12
DOIs	https://doi.org/10.3390/s16122068
Publication status	Published - 2016 Dec 5

Bibliographical note

Funding Information:
This research was supported by the NRFNational Research Foundation of Korea, funded by the Ministry of Education, Science, and Technology (No. 2016-900028, 2016-915744), and by grants from the Korea Health Technology R&D Project through the KHIDIKorea Health Industry Development Institute, funded by the Ministry of Health &Welfare, Korea (No. HI14C1135).

Publisher Copyright:
© 2016 by the authors; licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Information Systems
Instrumentation
Atomic and Molecular Physics, and Optics
Electrical and Electronic Engineering
Biochemistry

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title = "Electrical impedance monitoring of C2C12 myoblast differentiation on an indium tin oxide electrode",

abstract = "Electrical cell-substrate impedance sensing is increasingly being used for label-free and real-time monitoring of changes in cell morphology and number during cell growth, drug screening, and differentiation. In this study, we evaluated the feasibility of using ECIS to monitor C2C12 myoblast differentiation using a fabricated indium tin oxide (ITO) electrode-based chip. C2C12 myoblast differentiation on the ITO electrode was validated based on decreases in the mRNA level of MyoD and increases in the mRNA levels of myogenin and myosin heavy chain (MHC). Additionally, MHC expression and morphological changes in myoblasts differentiated on the ITO electrode were comparable to those in cells in the control culture dish. From the monitoring the integration of the resistance change at 21.5 kHz, the cell differentiation was label-free and real-time detectable in 30 h of differentiation (p < 0.05).",

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AU - Pyun, Jae Chul

AU - Choi, Jeong Woo

AU - Cho, Sungbo

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PY - 2016/12/5

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N2 - Electrical cell-substrate impedance sensing is increasingly being used for label-free and real-time monitoring of changes in cell morphology and number during cell growth, drug screening, and differentiation. In this study, we evaluated the feasibility of using ECIS to monitor C2C12 myoblast differentiation using a fabricated indium tin oxide (ITO) electrode-based chip. C2C12 myoblast differentiation on the ITO electrode was validated based on decreases in the mRNA level of MyoD and increases in the mRNA levels of myogenin and myosin heavy chain (MHC). Additionally, MHC expression and morphological changes in myoblasts differentiated on the ITO electrode were comparable to those in cells in the control culture dish. From the monitoring the integration of the resistance change at 21.5 kHz, the cell differentiation was label-free and real-time detectable in 30 h of differentiation (p < 0.05).

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Electrical impedance monitoring of C2C12 myoblast differentiation on an indium tin oxide electrode

Abstract

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