Efficient Self-Assembled MicroRNA Delivery System Consisting of Cholesterol-Conjugated MicroRNA and PEGylated Polycationic Polymer for Tumor Treatment

Byeonggeol Mun; Eunji Jang; Seungmin Han; Hye Young Son; Yuna Choi; Yong Min Huh; Seungjoo Haam

doi:10.1021/acsabm.9b00186

Efficient Self-Assembled MicroRNA Delivery System Consisting of Cholesterol-Conjugated MicroRNA and PEGylated Polycationic Polymer for Tumor Treatment

Byeonggeol Mun, Eunji Jang, Seungmin Han, Hye Young Son, Yuna Choi, Yong Min Huh, Seungjoo Haam

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

MicroRNA (miR), a key molecule involved in endogenous RNA interference, is a promising therapeutic agent. In vivo delivery of miR, however, is a major factor limiting its application because its polyanionic nature and vulnerability to breakdown make delivery of miR to targeted lesions difficult. To overcome these challenges, we developed a self-assembled miR delivery system consisting of cholesterol-conjugated miR and polyethylene glycol-grafted polyethylene imine. Nanosized complexes of miR with polyethylene imine, which protected miR and its delivery into targeted lesions in vivo, were successfully synthesized by polyethylene glycol grafting. The hydrophobicity of cholesterol improved the structural stability of the complex, preventing the loss of miR. Here, we report the preparation of this self-assembled complex. We examined the delivery efficiency and validated the therapeutic efficacy of the complex. In conclusion, our miR delivery system shows considerable potential for effective in vivo delivery of miR.

Original language	English
Pages (from-to)	2219-2228
Number of pages	10
Journal	ACS Applied Bio Materials
Volume	2
Issue number	5
DOIs	https://doi.org/10.1021/acsabm.9b00186
Publication status	Published - 2019 May 20

Bibliographical note

Funding Information:
This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science & ICT (NRF-2018M3A9E2022819), by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (NRF-2017M3A9G5083322 and NRF-2015M3A9D7029878), and by the Development of Platform Technology for Innovative Medical Measurements Program (KRISS-2018-GP2018-0018) from the Korea Research Institute of Standards and Science.

Publisher Copyright:
© 2019 American Chemical Society.

All Science Journal Classification (ASJC) codes

Biochemistry, medical
Chemistry(all)
Biomedical Engineering
Biomaterials

Access to Document

10.1021/acsabm.9b00186

Cite this

@article{63b32e9a4a834e62abb8cbcce52e4fe7,

title = "Efficient Self-Assembled MicroRNA Delivery System Consisting of Cholesterol-Conjugated MicroRNA and PEGylated Polycationic Polymer for Tumor Treatment",

abstract = "MicroRNA (miR), a key molecule involved in endogenous RNA interference, is a promising therapeutic agent. In vivo delivery of miR, however, is a major factor limiting its application because its polyanionic nature and vulnerability to breakdown make delivery of miR to targeted lesions difficult. To overcome these challenges, we developed a self-assembled miR delivery system consisting of cholesterol-conjugated miR and polyethylene glycol-grafted polyethylene imine. Nanosized complexes of miR with polyethylene imine, which protected miR and its delivery into targeted lesions in vivo, were successfully synthesized by polyethylene glycol grafting. The hydrophobicity of cholesterol improved the structural stability of the complex, preventing the loss of miR. Here, we report the preparation of this self-assembled complex. We examined the delivery efficiency and validated the therapeutic efficacy of the complex. In conclusion, our miR delivery system shows considerable potential for effective in vivo delivery of miR.",

author = "Byeonggeol Mun and Eunji Jang and Seungmin Han and Son, {Hye Young} and Yuna Choi and Huh, {Yong Min} and Seungjoo Haam",

note = "Funding Information: This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science & ICT (NRF-2018M3A9E2022819), by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (NRF-2017M3A9G5083322 and NRF-2015M3A9D7029878), and by the Development of Platform Technology for Innovative Medical Measurements Program (KRISS-2018-GP2018-0018) from the Korea Research Institute of Standards and Science. Publisher Copyright: {\textcopyright} 2019 American Chemical Society.",

year = "2019",

month = may,

day = "20",

doi = "10.1021/acsabm.9b00186",

language = "English",

volume = "2",

pages = "2219--2228",

journal = "ACS Applied Bio Materials",

issn = "2576-6422",

publisher = "American Chemical Society",

number = "5",

}

TY - JOUR

T1 - Efficient Self-Assembled MicroRNA Delivery System Consisting of Cholesterol-Conjugated MicroRNA and PEGylated Polycationic Polymer for Tumor Treatment

AU - Mun, Byeonggeol

AU - Jang, Eunji

AU - Han, Seungmin

AU - Son, Hye Young

AU - Choi, Yuna

AU - Huh, Yong Min

AU - Haam, Seungjoo

N1 - Funding Information: This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science & ICT (NRF-2018M3A9E2022819), by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (NRF-2017M3A9G5083322 and NRF-2015M3A9D7029878), and by the Development of Platform Technology for Innovative Medical Measurements Program (KRISS-2018-GP2018-0018) from the Korea Research Institute of Standards and Science. Publisher Copyright: © 2019 American Chemical Society.

PY - 2019/5/20

Y1 - 2019/5/20

N2 - MicroRNA (miR), a key molecule involved in endogenous RNA interference, is a promising therapeutic agent. In vivo delivery of miR, however, is a major factor limiting its application because its polyanionic nature and vulnerability to breakdown make delivery of miR to targeted lesions difficult. To overcome these challenges, we developed a self-assembled miR delivery system consisting of cholesterol-conjugated miR and polyethylene glycol-grafted polyethylene imine. Nanosized complexes of miR with polyethylene imine, which protected miR and its delivery into targeted lesions in vivo, were successfully synthesized by polyethylene glycol grafting. The hydrophobicity of cholesterol improved the structural stability of the complex, preventing the loss of miR. Here, we report the preparation of this self-assembled complex. We examined the delivery efficiency and validated the therapeutic efficacy of the complex. In conclusion, our miR delivery system shows considerable potential for effective in vivo delivery of miR.

AB - MicroRNA (miR), a key molecule involved in endogenous RNA interference, is a promising therapeutic agent. In vivo delivery of miR, however, is a major factor limiting its application because its polyanionic nature and vulnerability to breakdown make delivery of miR to targeted lesions difficult. To overcome these challenges, we developed a self-assembled miR delivery system consisting of cholesterol-conjugated miR and polyethylene glycol-grafted polyethylene imine. Nanosized complexes of miR with polyethylene imine, which protected miR and its delivery into targeted lesions in vivo, were successfully synthesized by polyethylene glycol grafting. The hydrophobicity of cholesterol improved the structural stability of the complex, preventing the loss of miR. Here, we report the preparation of this self-assembled complex. We examined the delivery efficiency and validated the therapeutic efficacy of the complex. In conclusion, our miR delivery system shows considerable potential for effective in vivo delivery of miR.

UR - http://www.scopus.com/inward/record.url?scp=85067969146&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85067969146&partnerID=8YFLogxK

U2 - 10.1021/acsabm.9b00186

DO - 10.1021/acsabm.9b00186

M3 - Article

AN - SCOPUS:85067969146

SN - 2576-6422

VL - 2

SP - 2219

EP - 2228

JO - ACS Applied Bio Materials

JF - ACS Applied Bio Materials

IS - 5

ER -

Efficient Self-Assembled MicroRNA Delivery System Consisting of Cholesterol-Conjugated MicroRNA and PEGylated Polycationic Polymer for Tumor Treatment

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this