Efficient bone regeneration induced by bone morphogenetic protein-2 released from apatite-coated collagen scaffolds

Hee Seok Yang, Wan Geun La, Jooyeon Park, Chang Sung Kim, Gun Il Im, Byung Soo Kim

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


Bone morphogenetic proteins (BMPs) are the most potent osteoinductive growth factors. Clinically utilized BMP-2 uses a type-I collagen scaffold as a carrier. Here we hypothesized that an apatite coating on a type-I collagen scaffold would prolong the BMP-2 release period and enhance bone regeneration in calvarial defects in mice. Apatite coating was achieved by incubating collagen scaffolds in simulated body fluid. BMP-2 release kinetics and bioactivity were evaluated by enzyme-linked immunosorbent assay and alkaline phosphatase activity measurement of cultured osteoblasts. Computed tomography and histomorphometry were performed eight weeks after various doses of BMP-2 were delivered to mouse calvarial defects using either non-modified or apatite-coated collagen scaffolds. Apatite-coated collagen scaffolds released 91.8 ± 11.5% of the loaded BMP-2 over 13 days in vitro, whereas non-modified collagen scaffolds released 98.3± 2.2% over the initial one day. The in vivo study showed that BMP-2 delivery with apatite-coated collagen scaffolds resulted in a significantly greater bone formation area and higher bone density than that with non-modified collagen scaffolds. This study suggests that simple apatite coating on collagen scaffolds can enhance the bone regeneration efficacy of BMP-2 released from collagen scaffolds.

Original languageEnglish
Pages (from-to)1659-1671
Number of pages13
JournalJournal of Biomaterials Science, Polymer Edition
Issue number13
Publication statusPublished - 2012

Bibliographical note

Funding Information:
H.S.Y. and W.-G.L. contributed equally to this work. This study was supported by a Grant 2009-0080769 from the National Research Foundation of Korea and a Grant A100443 from the Korea Health 21 R&D project, Ministry of Health and Welfare, Republic of Korea.

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering


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