Effects of V279F in the Lp-PLA2 gene on markers of oxidative stress and inflammation in Koreans

Jean Kyung Paik, Jey Sook Chae, Yangsoo Jang, Ji Young Kim, Oh Yoen Kim, Tae Sook Jeong, Sang Hyun Lee, Jong Ho Lee

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15 Citations (Scopus)


Background: A single nucleotide polymorphism (SNP), V279F, in the lipoprotein-associated phospholipase A2 (Lp-PLA2) gene is known to influence enzyme activity. It is unclear whether Lp-PLA2 exerts pro- or antiatherogenic effects in humans. We investigated the interplay between V279F, Lp-PLA2 activity, oxidative stress and inflammation. Methods: We genotyped 2914 healthy Koreans (43-79years) for the Lp-PLA2 V279F and measured anthropometric parameters, lipid profile, fatty acid composition, lipid peroxides, inflammatory markers and Lp-PLA2 levels. Results: Lp-PLA2 activity was 24% lower in V/F subjects (n=641) than in those with the V/V genotype (n=2227). Enzyme activity was undetectable in F/F subjects. Lp-PLA2 activity was positively correlated with LDL-cholesterol (r=0.134, P<0.001), ox-LDL (r=0.064, P<0.01), 8-epi-PGF (r=0.198, P<0.001), free fatty acid (r=0.082, P<0.001), and fibrinogen (r=0.112, P<0.01) levels. Additionally, ox-LDL, 8-epi-PGF, free fatty acid, and fibrinogen levels were positively correlated with hs-CRP. V279F was associated with LDL-cholesterol and arachidonic acid (AA) in serum phospholipid. F/F subjects had lower LDL-cholesterol than V/V subjects (V/V: 120.9±0.69, V/F: 119.4±1.26, F/F: 109.2±4.84mg/dl, P=0.025). A significant association between the F/F genotype and increasing AA in serum phospholipids was found in subjects with high LDL-cholesterol (≥130mg/dl) (P=0.003) but not in those with low LDL-cholesterol (<130mg/dl). F/F subjects in the high LDL-cholesterol group had CRP concentrations about three times higher than those with V/V or V/F genotypes (V/V: 1.25±0.09, V/F: 0.97±0.12, F/F: 3.20±0.88mg/dl, P<0.001). Conclusions: The recessive effects of Lp-PLA2 V279F on LDL-cholesterol and significant correlations between Lp-PLA2 activity and LDL-cholesterol, 8-epi-PGF and fibrinogen support a pro-oxidative or pro-atherogenic role for this enzyme. Paradoxically, the combination of the complete deficiency of Lp-PLA2 activity and high LDL-cholesterol enhanced lipid peroxidation and inflammation.

Original languageEnglish
Pages (from-to)486-493
Number of pages8
JournalClinica Chimica Acta
Issue number7-8
Publication statusPublished - 2010 Apr

Bibliographical note

Funding Information:
This work was funded by 1) National Research Laboratory grant # R0A-2005-000-10144-0 , Ministry of Science and Technology , Seoul, Korea, 2) Korea Health 21 R&D Projects, Ministry of Health & Welfare ( A000385 ), Seoul, Korea, 3) Korea Science and Engineering Foundation (KOSEF) grant ( 2009-0078457 ) , Ministry of Science and Technology , Seoul, Korea, 4) Korea Science and Engineering Foundation (KOSEF) grant ( M10642120002-06 N4212-00210 ) , Ministry of Science and Technology , Seoul, Korea.

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical


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