Effects of two fast-setting calcium-silicate cements on cell viability and angiogenic factor release in human pulp-derived cells

Chooryung J. Chung; Euiseong Kim; Minju Song; Jeong Won Park; Su Jung Shin

doi:10.1007/s10266-015-0194-5

Effects of two fast-setting calcium-silicate cements on cell viability and angiogenic factor release in human pulp-derived cells

Chooryung J. Chung, Euiseong Kim, Minju Song, Jeong Won Park, Su Jung Shin

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Mineral trioxide aggregate (MTA) is considered a pulp-capping agent of choice, but has the drawback of a long setting time. This study aimed to assess two different types of calcium-silicate cements as pulp-capping agents, by investigating their in vitro cytotoxicity and angiogenic effects in human pulp cells. ProRoot MTA, Endocem Zr, and Retro MTA were prepared as set or freshly mixed pellets. Human pulp-derived cells were grown in direct contact with these three cements, Dycal, or no cement, for 7 days. Initial cell attachment, viability, calcium release, and the levels of vascular endothelial growth factor (VEGF), angiogenin, and basic fibroblast growth factor (FGF-2) were evaluated statistically using a linear mixed model (P < 0.05). The biocompatibility of Retro MTA was similar to those of the control and ProRoot MTA. Endocem Zr groups showed fewer and more rounded cells after a 3-day culture; however, the initial cytotoxicity appeared transient. All test materials showed significant increases in calcium concentration compared with the control group (P < 0.05). VEGF and angiogenin levels in ProRoot MTA and Retro MTA groups were significantly higher than those in the Endocem Zr group (P < 0.05). FGF-2 levels were not significantly different between groups (P > 0.05). We demonstrate that Retro MTA, which has a short setting time, has similar biocompatibility and angiogenic effects on human pulp cells, and can therefore potentially be as effective in pulp capping as ProRoot MTA. Endocem Zr showed intermittent cytotoxicity and elicited lower levels of VEGF and angiogenin expression.

Original language	English
Pages (from-to)	143-151
Number of pages	9
Journal	Odontology
Volume	104
Issue number	2
DOIs	https://doi.org/10.1007/s10266-015-0194-5
Publication status	Published - 2016 May 1

Bibliographical note

Funding Information:
This study was supported by a Grant from the Korean Health Care Technology R&D Project, Ministry of Health Welfare & Family Affairs, Republic of Korea (A084458). The authors want to deliver a special thanks to Youngju Oh for her help about laboratory works and Hannah You for statistical analysis.

Publisher Copyright:
© 2015, The Society of The Nippon Dental University.

All Science Journal Classification (ASJC) codes

Dentistry(all)

Access to Document

10.1007/s10266-015-0194-5

Cite this

@article{cf9bbab802484a828a300c1cf335a0e9,

title = "Effects of two fast-setting calcium-silicate cements on cell viability and angiogenic factor release in human pulp-derived cells",

abstract = "Mineral trioxide aggregate (MTA) is considered a pulp-capping agent of choice, but has the drawback of a long setting time. This study aimed to assess two different types of calcium-silicate cements as pulp-capping agents, by investigating their in vitro cytotoxicity and angiogenic effects in human pulp cells. ProRoot MTA, Endocem Zr, and Retro MTA were prepared as set or freshly mixed pellets. Human pulp-derived cells were grown in direct contact with these three cements, Dycal, or no cement, for 7 days. Initial cell attachment, viability, calcium release, and the levels of vascular endothelial growth factor (VEGF), angiogenin, and basic fibroblast growth factor (FGF-2) were evaluated statistically using a linear mixed model (P < 0.05). The biocompatibility of Retro MTA was similar to those of the control and ProRoot MTA. Endocem Zr groups showed fewer and more rounded cells after a 3-day culture; however, the initial cytotoxicity appeared transient. All test materials showed significant increases in calcium concentration compared with the control group (P < 0.05). VEGF and angiogenin levels in ProRoot MTA and Retro MTA groups were significantly higher than those in the Endocem Zr group (P < 0.05). FGF-2 levels were not significantly different between groups (P > 0.05). We demonstrate that Retro MTA, which has a short setting time, has similar biocompatibility and angiogenic effects on human pulp cells, and can therefore potentially be as effective in pulp capping as ProRoot MTA. Endocem Zr showed intermittent cytotoxicity and elicited lower levels of VEGF and angiogenin expression.",

author = "Chung, {Chooryung J.} and Euiseong Kim and Minju Song and Park, {Jeong Won} and Shin, {Su Jung}",

note = "Funding Information: This study was supported by a Grant from the Korean Health Care Technology R&D Project, Ministry of Health Welfare & Family Affairs, Republic of Korea (A084458). The authors want to deliver a special thanks to Youngju Oh for her help about laboratory works and Hannah You for statistical analysis. Publisher Copyright: {\textcopyright} 2015, The Society of The Nippon Dental University.",

year = "2016",

month = may,

day = "1",

doi = "10.1007/s10266-015-0194-5",

language = "English",

volume = "104",

pages = "143--151",

journal = "Odontology",

issn = "1618-1247",

publisher = "Springer Japan",

number = "2",

}

TY - JOUR

T1 - Effects of two fast-setting calcium-silicate cements on cell viability and angiogenic factor release in human pulp-derived cells

AU - Chung, Chooryung J.

AU - Kim, Euiseong

AU - Song, Minju

AU - Park, Jeong Won

AU - Shin, Su Jung

N1 - Funding Information: This study was supported by a Grant from the Korean Health Care Technology R&D Project, Ministry of Health Welfare & Family Affairs, Republic of Korea (A084458). The authors want to deliver a special thanks to Youngju Oh for her help about laboratory works and Hannah You for statistical analysis. Publisher Copyright: © 2015, The Society of The Nippon Dental University.

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Mineral trioxide aggregate (MTA) is considered a pulp-capping agent of choice, but has the drawback of a long setting time. This study aimed to assess two different types of calcium-silicate cements as pulp-capping agents, by investigating their in vitro cytotoxicity and angiogenic effects in human pulp cells. ProRoot MTA, Endocem Zr, and Retro MTA were prepared as set or freshly mixed pellets. Human pulp-derived cells were grown in direct contact with these three cements, Dycal, or no cement, for 7 days. Initial cell attachment, viability, calcium release, and the levels of vascular endothelial growth factor (VEGF), angiogenin, and basic fibroblast growth factor (FGF-2) were evaluated statistically using a linear mixed model (P < 0.05). The biocompatibility of Retro MTA was similar to those of the control and ProRoot MTA. Endocem Zr groups showed fewer and more rounded cells after a 3-day culture; however, the initial cytotoxicity appeared transient. All test materials showed significant increases in calcium concentration compared with the control group (P < 0.05). VEGF and angiogenin levels in ProRoot MTA and Retro MTA groups were significantly higher than those in the Endocem Zr group (P < 0.05). FGF-2 levels were not significantly different between groups (P > 0.05). We demonstrate that Retro MTA, which has a short setting time, has similar biocompatibility and angiogenic effects on human pulp cells, and can therefore potentially be as effective in pulp capping as ProRoot MTA. Endocem Zr showed intermittent cytotoxicity and elicited lower levels of VEGF and angiogenin expression.

AB - Mineral trioxide aggregate (MTA) is considered a pulp-capping agent of choice, but has the drawback of a long setting time. This study aimed to assess two different types of calcium-silicate cements as pulp-capping agents, by investigating their in vitro cytotoxicity and angiogenic effects in human pulp cells. ProRoot MTA, Endocem Zr, and Retro MTA were prepared as set or freshly mixed pellets. Human pulp-derived cells were grown in direct contact with these three cements, Dycal, or no cement, for 7 days. Initial cell attachment, viability, calcium release, and the levels of vascular endothelial growth factor (VEGF), angiogenin, and basic fibroblast growth factor (FGF-2) were evaluated statistically using a linear mixed model (P < 0.05). The biocompatibility of Retro MTA was similar to those of the control and ProRoot MTA. Endocem Zr groups showed fewer and more rounded cells after a 3-day culture; however, the initial cytotoxicity appeared transient. All test materials showed significant increases in calcium concentration compared with the control group (P < 0.05). VEGF and angiogenin levels in ProRoot MTA and Retro MTA groups were significantly higher than those in the Endocem Zr group (P < 0.05). FGF-2 levels were not significantly different between groups (P > 0.05). We demonstrate that Retro MTA, which has a short setting time, has similar biocompatibility and angiogenic effects on human pulp cells, and can therefore potentially be as effective in pulp capping as ProRoot MTA. Endocem Zr showed intermittent cytotoxicity and elicited lower levels of VEGF and angiogenin expression.

UR - http://www.scopus.com/inward/record.url?scp=84921508612&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84921508612&partnerID=8YFLogxK

U2 - 10.1007/s10266-015-0194-5

DO - 10.1007/s10266-015-0194-5

M3 - Article

C2 - 25596932

AN - SCOPUS:84921508612

SN - 1618-1247

VL - 104

SP - 143

EP - 151

JO - Odontology

JF - Odontology

IS - 2

ER -

Effects of two fast-setting calcium-silicate cements on cell viability and angiogenic factor release in human pulp-derived cells

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this