Effects of sterols on the development and aging of Caenorhabditis elegans.

Eun Young Lee, Pan Young Jeong, Sun Young Kim, Yhong Hee Shim, David J. Chitwood, Young Ki Paik

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Although Caenorhabditis elegans lacks several components of the de novo sterol biosynthetic pathway, it requires sterols as essential nutrients. Supplemental cholesterol undergoes extensive enzymatic modification in C. elegans to form certain sterols of unknown function. Since sterol metabolism in C. elegans differs from that in other species, such as mammals and yeast, it is important to examine how sterols regulate worm physiology. To examine the functions of sterols in C. elegans, a sterol-feeding experiment was carried out and several critical parameters, such as brood size, growth rate, and life span, were measured. In addition, the change in lipid distribution in C. elegans can be both qualitatively and quantitatively determined by various methods, including staining and chromatographic techniques. Taken together, the effects of sterols on C. elegans are very prominent and can be easily assessed using the techniques described here.

Original languageEnglish
Pages (from-to)167-179
Number of pages13
JournalMethods in molecular biology (Clifton, N.J.)
Publication statusPublished - 2009

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics


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