Abstract
An in vitro neural network can provide a model to investigate the signaling processes that regulate the body functions. Using polydimethylsiloxane (PDMS) multilayer micro-structures to construct a neural network, the bonding between the layers can be improved by modulation of the curing ratio. In this study, we found that as the curing ratio increased from 10:1 to 10:4, the contact angle decreased from 111.69° to 102.08° and the surface energy and roughness increased. For adhesion and proliferation, the hippocampal primary neural cells preferred a PDMS surface with a 10:1 curing ratio to other surfaces. In addition, 3D PDMS micro-pyramid array allows the primary hippocampal neuron to pattern a network without any surface treatment and the network was verified by immunocytochemistry. These results may suggest the optimum PDMS curing ratio to apply in constructing a cell device for in vitro neural network formation as well as the potential of 3D structure fabrication which allows us to construct neural network without any surface treatment.
| Original language | English |
|---|---|
| Pages (from-to) | e294-e297 |
| Journal | Current Applied Physics |
| Volume | 9 |
| Issue number | 4 SUPPL. |
| DOIs | |
| Publication status | Published - 2009 Jul |
Bibliographical note
Funding Information:This work was supported by the National Core Research Center (NCRC) for Nanomedical Technology of the Korea Science and Engineering Foundation (Grant No. R15-2004-024-01001-0), Seoul Research and Business Development (Seoul R&BD Program, 10816) and “System IC 2010” project of the Korea ministry of Commerce, Industry and Energy.
All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Physics and Astronomy(all)