Effects of dissolving microneedle fabrication parameters on the activity of encapsulated lysozyme

Shayan Fakhraei Lahiji; Yoojung Jang; Yonghao Ma; Manita Dangol; Huisuk Yang; Mingyu Jang; Hyungil Jung

doi:10.1016/j.ejps.2018.03.003

Effects of dissolving microneedle fabrication parameters on the activity of encapsulated lysozyme

Shayan Fakhraei Lahiji, Yoojung Jang, Yonghao Ma, Manita Dangol, Huisuk Yang, Mingyu Jang, Hyungil Jung

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Dissolving microneedle (DMN) is referred to a microscale needle that encapsulates drug(s) within a biodegradable polymer matrix and delivers it into the skin in a minimally invasive manner. Although vast majority of studies have emphasized DMN as an efficient drug delivery system, the activity of DMN-encapsulated proteins or antigens can be significantly affected due to a series of thermal, physical and chemical stress factors during DMN fabrication process and storage period. The objective of this study is to evaluate the effects of DMN fabrication parameters including polymer type, polymer concentration, fabrication and storage temperature, and drying conditions on the activity of the encapsulated therapeutic proteins by employing lysozyme (LYS) as a model protein. Our results indicate that a combination of low temperature fabrication, mild drying condition, specific polymer concentration, and addition of protein stabilizer can maintain the activity of encapsulated LYS up to 99.8 ± 3.8%. Overall, findings of this study highlight the importance of optimizing DMN fabrication parameters and paves way for the commercialization of an efficient delivery system for therapeutics.

Original language	English
Pages (from-to)	290-296
Number of pages	7
Journal	European Journal of Pharmaceutical Sciences
Volume	117
DOIs	https://doi.org/10.1016/j.ejps.2018.03.003
Publication status	Published - 2018 May 30

Bibliographical note

Funding Information:
Authors would like to thank Suyong Kim and Inyoung Huh for their assistance during this research. This work was supported equally by the R&D program of MSIP/COMPA ( 2016K000225 , Development of minimal pain multi-micro lancets for one-touchsmart diagnostic sensor) and by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI16C0625 ).

Funding Information:
Authors would like to thank Suyong Kim and Inyoung Huh for their assistance during this research. This work was supported equally by the R&D program of MSIP/COMPA (2016K000225, Development of minimal pain multi-micro lancets for one-touchsmart diagnostic sensor) and by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI16C0625).

Publisher Copyright:
© 2018

All Science Journal Classification (ASJC) codes

Pharmaceutical Science

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10.1016/j.ejps.2018.03.003

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title = "Effects of dissolving microneedle fabrication parameters on the activity of encapsulated lysozyme",

abstract = "Dissolving microneedle (DMN) is referred to a microscale needle that encapsulates drug(s) within a biodegradable polymer matrix and delivers it into the skin in a minimally invasive manner. Although vast majority of studies have emphasized DMN as an efficient drug delivery system, the activity of DMN-encapsulated proteins or antigens can be significantly affected due to a series of thermal, physical and chemical stress factors during DMN fabrication process and storage period. The objective of this study is to evaluate the effects of DMN fabrication parameters including polymer type, polymer concentration, fabrication and storage temperature, and drying conditions on the activity of the encapsulated therapeutic proteins by employing lysozyme (LYS) as a model protein. Our results indicate that a combination of low temperature fabrication, mild drying condition, specific polymer concentration, and addition of protein stabilizer can maintain the activity of encapsulated LYS up to 99.8 ± 3.8%. Overall, findings of this study highlight the importance of optimizing DMN fabrication parameters and paves way for the commercialization of an efficient delivery system for therapeutics.",

author = "{Fakhraei Lahiji}, Shayan and Yoojung Jang and Yonghao Ma and Manita Dangol and Huisuk Yang and Mingyu Jang and Hyungil Jung",

note = "Funding Information: Authors would like to thank Suyong Kim and Inyoung Huh for their assistance during this research. This work was supported equally by the R&D program of MSIP/COMPA ( 2016K000225 , Development of minimal pain multi-micro lancets for one-touchsmart diagnostic sensor) and by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI16C0625 ). Funding Information: Authors would like to thank Suyong Kim and Inyoung Huh for their assistance during this research. This work was supported equally by the R&D program of MSIP/COMPA (2016K000225, Development of minimal pain multi-micro lancets for one-touchsmart diagnostic sensor) and by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI16C0625). Publisher Copyright: {\textcopyright} 2018",

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AU - Ma, Yonghao

AU - Dangol, Manita

AU - Yang, Huisuk

AU - Jang, Mingyu

AU - Jung, Hyungil

N1 - Funding Information: Authors would like to thank Suyong Kim and Inyoung Huh for their assistance during this research. This work was supported equally by the R&D program of MSIP/COMPA ( 2016K000225 , Development of minimal pain multi-micro lancets for one-touchsmart diagnostic sensor) and by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI16C0625 ). Funding Information: Authors would like to thank Suyong Kim and Inyoung Huh for their assistance during this research. This work was supported equally by the R&D program of MSIP/COMPA (2016K000225, Development of minimal pain multi-micro lancets for one-touchsmart diagnostic sensor) and by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI16C0625). Publisher Copyright: © 2018

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N2 - Dissolving microneedle (DMN) is referred to a microscale needle that encapsulates drug(s) within a biodegradable polymer matrix and delivers it into the skin in a minimally invasive manner. Although vast majority of studies have emphasized DMN as an efficient drug delivery system, the activity of DMN-encapsulated proteins or antigens can be significantly affected due to a series of thermal, physical and chemical stress factors during DMN fabrication process and storage period. The objective of this study is to evaluate the effects of DMN fabrication parameters including polymer type, polymer concentration, fabrication and storage temperature, and drying conditions on the activity of the encapsulated therapeutic proteins by employing lysozyme (LYS) as a model protein. Our results indicate that a combination of low temperature fabrication, mild drying condition, specific polymer concentration, and addition of protein stabilizer can maintain the activity of encapsulated LYS up to 99.8 ± 3.8%. Overall, findings of this study highlight the importance of optimizing DMN fabrication parameters and paves way for the commercialization of an efficient delivery system for therapeutics.

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Effects of dissolving microneedle fabrication parameters on the activity of encapsulated lysozyme

Abstract

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