TY - JOUR
T1 - Effects of α-melanocyte-stimulating hormone on calcium concentration in SZ95 sebocytes
AU - Whang, Sung Won
AU - Lee, Sang Eun
AU - Kim, Ji Min
AU - Kim, Hyun Jung
AU - Jeong, Se Kyoo
AU - Zouboulis, Christos C.
AU - Seo, Jeong Taek
AU - Lee, Seung Hun
PY - 2011/1
Y1 - 2011/1
N2 - Melanocortins have been implicated in human sebum secretion for a long time. However, the signalling pathways of α-melanocyte-stimulating hormone (α-MSH) in human sebocytes expressing melanocortin receptors (MC-Rs) are still poorly understood. Because calcium ions play a central role in MC-R signalling, we investigated whether α-MSH affects calcium signalling in the immortalized human sebocyte cell line SZ95. In addition, we investigated the impact of α-MSH on MC-1R expression and lipid synthesis in these cells. α-MSH increased intracellular calcium levels. α-MSH-mediated calcium mobilization originated from intracellular calcium stores and was mediated by inositol triphosphate. Moreover, α-MSH increased MC-1R immunoreactivity and lipid synthesis in SZ95 sebocytes in the presence of testosterone. Our data demonstrate that α-MSH in human sebocytes controls a key cellular signalling pathway, the calcium ion response, which may coordinate MC-1R-mediated sebum secretion.
AB - Melanocortins have been implicated in human sebum secretion for a long time. However, the signalling pathways of α-melanocyte-stimulating hormone (α-MSH) in human sebocytes expressing melanocortin receptors (MC-Rs) are still poorly understood. Because calcium ions play a central role in MC-R signalling, we investigated whether α-MSH affects calcium signalling in the immortalized human sebocyte cell line SZ95. In addition, we investigated the impact of α-MSH on MC-1R expression and lipid synthesis in these cells. α-MSH increased intracellular calcium levels. α-MSH-mediated calcium mobilization originated from intracellular calcium stores and was mediated by inositol triphosphate. Moreover, α-MSH increased MC-1R immunoreactivity and lipid synthesis in SZ95 sebocytes in the presence of testosterone. Our data demonstrate that α-MSH in human sebocytes controls a key cellular signalling pathway, the calcium ion response, which may coordinate MC-1R-mediated sebum secretion.
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U2 - 10.1111/j.1600-0625.2010.01199.x
DO - 10.1111/j.1600-0625.2010.01199.x
M3 - Article
C2 - 21158935
AN - SCOPUS:78650216364
SN - 0906-6705
VL - 20
SP - 19
EP - 23
JO - Experimental dermatology
JF - Experimental dermatology
IS - 1
ER -