Effective seeding strategy using flat type poly (vinyl alcohol) cryogel for anammox enrichment

Minkyu Choi; Kyungjin Cho; Seockheon Lee; Yun Chul Chung; Joonhong Park; Hyokwan Bae

doi:10.1016/j.chemosphere.2018.04.055

Effective seeding strategy using flat type poly (vinyl alcohol) cryogel for anammox enrichment

Minkyu Choi, Kyungjin Cho, Seockheon Lee, Yun Chul Chung, Joonhong Park, Hyokwan Bae

Department of Civil and Environmental Engineering

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

In this study, anammox enrichment reactors were operated using flat type poly (vinyl alcohol) cryogel (cryoPVAG) with precultured anammox bacteria (PAB) and activated sludge (AS) from an anoxic tack of the A2O process to evaluate the effect of different seeding sources on anammox enrichment. In addition, cryoPVAGs with different thicknesses (1, 2, and 3 mm) were used to investigate the effects of the thickness on anammox enrichment. The regression analysis with a modified Gompertz model showed that the start-up period of the anammox enrichment using PAB inoculum was approximately 14 days earlier than that of AS inoculum at a nitrogen loading rate of approximately 1 kg-N m⁻³ day⁻¹. Substrate diffusion was limited in 3-mm cryoPVAG with respect to trend in nitrogen removal rate. Quantitative PCR analysis indicated that in the initial phase, the 16S rRNA gene copy numbers of anammox microorganism in cryoPVAG were significantly different according to the seeding source, but finally converged to a similar level after anammox enrichment. The anammox reaction was initially promoted by cryoPVAG. Next, anammox biomass detached from cryoPVAG and enriched in the bulk phase to maximize NRR. Illumina MiSeq sequencing revealed that Candidatus Brocadia sinica led to the active anammox reaction, and its relative abundance decreased with increasing gel thickness.

Original language	English
Pages (from-to)	88-97
Number of pages	10
Journal	Chemosphere
Volume	205
DOIs	https://doi.org/10.1016/j.chemosphere.2018.04.055
Publication status	Published - 2018 Aug

Bibliographical note

Funding Information:
This work was supported by the Korea Institute of Science and Technology ( 2E27102 ) and the National Research Foundation of Korea grant funded by the Korea government (MSIP) (No. 2011-0030040 ).

Publisher Copyright:
© 2018 Elsevier Ltd

All Science Journal Classification (ASJC) codes

Public Health, Environmental and Occupational Health
Pollution
Chemistry(all)
Health, Toxicology and Mutagenesis
Environmental Engineering
Environmental Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.chemosphere.2018.04.055

Cite this

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title = "Effective seeding strategy using flat type poly (vinyl alcohol) cryogel for anammox enrichment",

abstract = "In this study, anammox enrichment reactors were operated using flat type poly (vinyl alcohol) cryogel (cryoPVAG) with precultured anammox bacteria (PAB) and activated sludge (AS) from an anoxic tack of the A2O process to evaluate the effect of different seeding sources on anammox enrichment. In addition, cryoPVAGs with different thicknesses (1, 2, and 3 mm) were used to investigate the effects of the thickness on anammox enrichment. The regression analysis with a modified Gompertz model showed that the start-up period of the anammox enrichment using PAB inoculum was approximately 14 days earlier than that of AS inoculum at a nitrogen loading rate of approximately 1 kg-N m−3 day−1. Substrate diffusion was limited in 3-mm cryoPVAG with respect to trend in nitrogen removal rate. Quantitative PCR analysis indicated that in the initial phase, the 16S rRNA gene copy numbers of anammox microorganism in cryoPVAG were significantly different according to the seeding source, but finally converged to a similar level after anammox enrichment. The anammox reaction was initially promoted by cryoPVAG. Next, anammox biomass detached from cryoPVAG and enriched in the bulk phase to maximize NRR. Illumina MiSeq sequencing revealed that Candidatus Brocadia sinica led to the active anammox reaction, and its relative abundance decreased with increasing gel thickness.",

author = "Minkyu Choi and Kyungjin Cho and Seockheon Lee and Chung, {Yun Chul} and Joonhong Park and Hyokwan Bae",

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AU - Choi, Minkyu

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AU - Park, Joonhong

AU - Bae, Hyokwan

N1 - Funding Information: This work was supported by the Korea Institute of Science and Technology ( 2E27102 ) and the National Research Foundation of Korea grant funded by the Korea government (MSIP) (No. 2011-0030040 ). Publisher Copyright: © 2018 Elsevier Ltd

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Effective seeding strategy using flat type poly (vinyl alcohol) cryogel for anammox enrichment

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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