Effect of radiopaque Portland cement on mineralization in human dental pulp cells

Kyung San Min; Sang Im Lee; Yoon Lee; Eun Cheol Kim

doi:10.1016/j.tripleo.2009.05.022

Effect of radiopaque Portland cement on mineralization in human dental pulp cells

Kyung San Min, Sang Im Lee, Yoon Lee, Eun Cheol Kim

Dentistry

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Objective: The aim of this study was to investigate whether radiopaque Portland cement (RPC) facilitates the mineralization process in human dental pulp cells (HDPCs) compared with pure Portland cement (PC). Study design: Under a scanning electron microscope (SEM), cellular morphology was evaluated. Alkaline phosphatase (ALP) activity was analyzed, and nodule formation was assessed by performing Alizarin Red S staining. In addition, the mRNA expressions of mineralization-related proteins were evaluated by performing a real-time polymerase chain reaction. Results: On SEM evaluation, healthy HDPCs were found adhering to the surfaces of PC and RPC. The ALP activity increased in the PC and RPC groups compared with the control group at 1 day. Alizarin Red stain increased in the PC and RPC groups compared with the control group at 2 and 3 weeks. The mRNA expression of dentin sialophosphoprotein increased at 14 days in the PC and RPC groups. Conclusions: These results show that PC and RPC have similar effects in terms of mineralization and suggest that RPC also has the potential to be used as a clinically suitable pulp-capping material.

Original language	English
Pages (from-to)	e82-e86
Journal	Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology
Volume	108
Issue number	4
DOIs	https://doi.org/10.1016/j.tripleo.2009.05.022
Publication status	Published - 2009 Oct

Bibliographical note

Funding Information:
This paper was supported by Wonkwang University in 2009.

All Science Journal Classification (ASJC) codes

Surgery
Oral Surgery
Otorhinolaryngology
Dentistry(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.tripleo.2009.05.022

Cite this

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title = "Effect of radiopaque Portland cement on mineralization in human dental pulp cells",

abstract = "Objective: The aim of this study was to investigate whether radiopaque Portland cement (RPC) facilitates the mineralization process in human dental pulp cells (HDPCs) compared with pure Portland cement (PC). Study design: Under a scanning electron microscope (SEM), cellular morphology was evaluated. Alkaline phosphatase (ALP) activity was analyzed, and nodule formation was assessed by performing Alizarin Red S staining. In addition, the mRNA expressions of mineralization-related proteins were evaluated by performing a real-time polymerase chain reaction. Results: On SEM evaluation, healthy HDPCs were found adhering to the surfaces of PC and RPC. The ALP activity increased in the PC and RPC groups compared with the control group at 1 day. Alizarin Red stain increased in the PC and RPC groups compared with the control group at 2 and 3 weeks. The mRNA expression of dentin sialophosphoprotein increased at 14 days in the PC and RPC groups. Conclusions: These results show that PC and RPC have similar effects in terms of mineralization and suggest that RPC also has the potential to be used as a clinically suitable pulp-capping material.",

author = "Min, {Kyung San} and Lee, {Sang Im} and Yoon Lee and Kim, {Eun Cheol}",

note = "Funding Information: This paper was supported by Wonkwang University in 2009. ",

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AU - Lee, Sang Im

AU - Lee, Yoon

AU - Kim, Eun Cheol

N1 - Funding Information: This paper was supported by Wonkwang University in 2009.

PY - 2009/10

Y1 - 2009/10

N2 - Objective: The aim of this study was to investigate whether radiopaque Portland cement (RPC) facilitates the mineralization process in human dental pulp cells (HDPCs) compared with pure Portland cement (PC). Study design: Under a scanning electron microscope (SEM), cellular morphology was evaluated. Alkaline phosphatase (ALP) activity was analyzed, and nodule formation was assessed by performing Alizarin Red S staining. In addition, the mRNA expressions of mineralization-related proteins were evaluated by performing a real-time polymerase chain reaction. Results: On SEM evaluation, healthy HDPCs were found adhering to the surfaces of PC and RPC. The ALP activity increased in the PC and RPC groups compared with the control group at 1 day. Alizarin Red stain increased in the PC and RPC groups compared with the control group at 2 and 3 weeks. The mRNA expression of dentin sialophosphoprotein increased at 14 days in the PC and RPC groups. Conclusions: These results show that PC and RPC have similar effects in terms of mineralization and suggest that RPC also has the potential to be used as a clinically suitable pulp-capping material.

AB - Objective: The aim of this study was to investigate whether radiopaque Portland cement (RPC) facilitates the mineralization process in human dental pulp cells (HDPCs) compared with pure Portland cement (PC). Study design: Under a scanning electron microscope (SEM), cellular morphology was evaluated. Alkaline phosphatase (ALP) activity was analyzed, and nodule formation was assessed by performing Alizarin Red S staining. In addition, the mRNA expressions of mineralization-related proteins were evaluated by performing a real-time polymerase chain reaction. Results: On SEM evaluation, healthy HDPCs were found adhering to the surfaces of PC and RPC. The ALP activity increased in the PC and RPC groups compared with the control group at 1 day. Alizarin Red stain increased in the PC and RPC groups compared with the control group at 2 and 3 weeks. The mRNA expression of dentin sialophosphoprotein increased at 14 days in the PC and RPC groups. Conclusions: These results show that PC and RPC have similar effects in terms of mineralization and suggest that RPC also has the potential to be used as a clinically suitable pulp-capping material.

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Effect of radiopaque Portland cement on mineralization in human dental pulp cells

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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