TY - JOUR
T1 - Effect of nitric oxide on hydrogen peroxide-induced damage in isolated rabbit gastric glands
AU - Kim, Hyeyoung
AU - Kim, Kyung Hwan
PY - 1998/12
Y1 - 1998/12
N2 - The present study aims at investigating the effects of nitric oxide (NO) on hydrogen peroxide (H2O2)-induced damage in isolated rabbit gastric glands. NO synthesis modulators such as L-arginine and N(G)-nitro-L-arginine methyl ester (L-NAME) and an NO donor, sodium nitroprusside, were added to isolated rabbit gastric glands exposed to H2O2, generated by glucose oxidase acting on β-D-glucose. As a result, glucose/glucose oxidase caused an increase in lipid peroxide production and decreases in reduced glutathione (GSH) content, GSH peroxidase activity, nitrite release, and mucus secretion in gastric glands. The alterations in lipid peroxide production, GSH content, and mucus secretion were prevented by pretreatment with L-arginine, a substrate for NO synthase and sodium nitroprusside, but not by L-NAME. In conclusion, NO protects gastric glands from H2O2 by inhibiting lipid peroxidation and maintaining cellular GSH content and mucus secretion.
AB - The present study aims at investigating the effects of nitric oxide (NO) on hydrogen peroxide (H2O2)-induced damage in isolated rabbit gastric glands. NO synthesis modulators such as L-arginine and N(G)-nitro-L-arginine methyl ester (L-NAME) and an NO donor, sodium nitroprusside, were added to isolated rabbit gastric glands exposed to H2O2, generated by glucose oxidase acting on β-D-glucose. As a result, glucose/glucose oxidase caused an increase in lipid peroxide production and decreases in reduced glutathione (GSH) content, GSH peroxidase activity, nitrite release, and mucus secretion in gastric glands. The alterations in lipid peroxide production, GSH content, and mucus secretion were prevented by pretreatment with L-arginine, a substrate for NO synthase and sodium nitroprusside, but not by L-NAME. In conclusion, NO protects gastric glands from H2O2 by inhibiting lipid peroxidation and maintaining cellular GSH content and mucus secretion.
UR - http://www.scopus.com/inward/record.url?scp=0031739221&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031739221&partnerID=8YFLogxK
U2 - 10.1159/000028258
DO - 10.1159/000028258
M3 - Article
C2 - 9792973
AN - SCOPUS:0031739221
SN - 0031-7012
VL - 57
SP - 323
EP - 330
JO - Pharmacology
JF - Pharmacology
IS - 6
ER -