Effect of FGF-2 on collagen tissue regeneration by human vertebral bone marrow stem cells

Dong Soo Park, Jung Chul Park, Jung Seok Lee, Tae Wan Kim, Ki Joon Kim, Byung Joo Jung, Eun Kyung Shim, Eun Young Choi, So Yon Park, Kyoo Sung Cho, Chang Sung Kim

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12 Citations (Scopus)


The effects of fibroblast growth factor-2 (FGF-2) on collagen tissue regeneration by human bone marrow stem cells (hBMSCs) were investigated. hBMSCs were isolated from human vertebral body bone marrow during vertebral surgery and a population of hBMSCs with the characteristics of mesenchymal stem cells was observed. The FGF-2 treatment (5 ng/mL) affected on the colony-forming efficiency, proliferation, and in vitro differentiation of hBMSCs. Insoluble/soluble collagen and hydroxyproline synthesis was significantly enhanced in hBMSCs expanded with FGF-2 and the treatment of FGF-2 caused a reduction in the mRNA expression of collagen type I, but an increase of collagen types II and III along with lysyl oxidase family genes. Collagen formation was also examined using an in vivo assay model by transplanting hBMSCs into immunocompromised mice (n=4) and the histologic and immunohistochemical results revealed that significantly more collagen with a well-organized structure was formed by FGF-2-treated hBMSCs at 8 weeks posttransplantation (P<0.05). The DNA microarray assay demonstrated that genes related to extracellular matrix formation were significantly upregulated. To elucidate the underlying mechanism, chemical inhibitors against extracellular-signal-regulated kinase (ERK) and phosphoinositide 3-kinase (PI3K) were treated and following downstream expression was observed. Collectively, FGF-2 facilitated the collagen-producing potency of hBMSCs both in vitro and in vivo, rendering them more suitable for use in collagen regeneration in the clinical field.

Original languageEnglish
Pages (from-to)228-243
Number of pages16
JournalStem Cells and Development
Issue number2
Publication statusPublished - 2015 Jan 15

Bibliographical note

Publisher Copyright:
© 2015 Mary Ann Liebert, Inc.

All Science Journal Classification (ASJC) codes

  • Hematology
  • Developmental Biology
  • Cell Biology


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