Effect of dissolution temperature on the structures of sodium hyaluronate by flow field-flow fractionation/multiangle light scattering

Heejeong Lee; Il Hwan Cho; Myeong Hee Moon

doi:10.1016/j.chroma.2006.07.049

Effect of dissolution temperature on the structures of sodium hyaluronate by flow field-flow fractionation/multiangle light scattering

Heejeong Lee, Il Hwan Cho, Myeong Hee Moon

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Molecular weight distribution (MWD) and structural deformation of ultrahigh molecular weight (MW) sodium hylaluronate (10⁵-10⁸ g/mol) were studied under different sample dissolution temperature conditions, using on-line flow field-flow fractionation (FlFFF) and multiangle light scattering (MALS). Sodium hyaluronate (NaHA) materials from sarcoma fluid have been studied by dissolving them in water at three different temperature conditions (5 °C, 50 °C, and 90 °C). Frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF), with field programming, was utilized for the separation of NaHA by MW, and on-line observation of light scattering of fractionated NaHA by MALS was performed in order to determine the MWD and molecular conformation. In these experiments, NaHA molecules exhibited an extended structure from a formerly rather compact geometry when the dissolving temperature was raised to 90 °C. This study also showed a clear difference in the MWD of NaHA when a preliminary filtration process was applied.

Original language	English
Pages (from-to)	185-191
Number of pages	7
Journal	Journal of Chromatography A
Volume	1131
Issue number	1-2
DOIs	https://doi.org/10.1016/j.chroma.2006.07.049
Publication status	Published - 2006 Oct 27

Bibliographical note

Funding Information:
This study was supported by the Korea Research Foundation Grant (KRF-2004-015-C00348) and, also, partly by a grant from the Korea Science and Engineering Foundation through the Center for Bioactive Molecular Hybrids (CBMH) at Yonsei University.

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/j.chroma.2006.07.049

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title = "Effect of dissolution temperature on the structures of sodium hyaluronate by flow field-flow fractionation/multiangle light scattering",

abstract = "Molecular weight distribution (MWD) and structural deformation of ultrahigh molecular weight (MW) sodium hylaluronate (105-108 g/mol) were studied under different sample dissolution temperature conditions, using on-line flow field-flow fractionation (FlFFF) and multiangle light scattering (MALS). Sodium hyaluronate (NaHA) materials from sarcoma fluid have been studied by dissolving them in water at three different temperature conditions (5 °C, 50 °C, and 90 °C). Frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF), with field programming, was utilized for the separation of NaHA by MW, and on-line observation of light scattering of fractionated NaHA by MALS was performed in order to determine the MWD and molecular conformation. In these experiments, NaHA molecules exhibited an extended structure from a formerly rather compact geometry when the dissolving temperature was raised to 90 °C. This study also showed a clear difference in the MWD of NaHA when a preliminary filtration process was applied.",

author = "Heejeong Lee and Cho, {Il Hwan} and Moon, {Myeong Hee}",

note = "Funding Information: This study was supported by the Korea Research Foundation Grant (KRF-2004-015-C00348) and, also, partly by a grant from the Korea Science and Engineering Foundation through the Center for Bioactive Molecular Hybrids (CBMH) at Yonsei University.",

year = "2006",

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doi = "10.1016/j.chroma.2006.07.049",

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AU - Cho, Il Hwan

AU - Moon, Myeong Hee

N1 - Funding Information: This study was supported by the Korea Research Foundation Grant (KRF-2004-015-C00348) and, also, partly by a grant from the Korea Science and Engineering Foundation through the Center for Bioactive Molecular Hybrids (CBMH) at Yonsei University.

PY - 2006/10/27

Y1 - 2006/10/27

N2 - Molecular weight distribution (MWD) and structural deformation of ultrahigh molecular weight (MW) sodium hylaluronate (105-108 g/mol) were studied under different sample dissolution temperature conditions, using on-line flow field-flow fractionation (FlFFF) and multiangle light scattering (MALS). Sodium hyaluronate (NaHA) materials from sarcoma fluid have been studied by dissolving them in water at three different temperature conditions (5 °C, 50 °C, and 90 °C). Frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF), with field programming, was utilized for the separation of NaHA by MW, and on-line observation of light scattering of fractionated NaHA by MALS was performed in order to determine the MWD and molecular conformation. In these experiments, NaHA molecules exhibited an extended structure from a formerly rather compact geometry when the dissolving temperature was raised to 90 °C. This study also showed a clear difference in the MWD of NaHA when a preliminary filtration process was applied.

AB - Molecular weight distribution (MWD) and structural deformation of ultrahigh molecular weight (MW) sodium hylaluronate (105-108 g/mol) were studied under different sample dissolution temperature conditions, using on-line flow field-flow fractionation (FlFFF) and multiangle light scattering (MALS). Sodium hyaluronate (NaHA) materials from sarcoma fluid have been studied by dissolving them in water at three different temperature conditions (5 °C, 50 °C, and 90 °C). Frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF), with field programming, was utilized for the separation of NaHA by MW, and on-line observation of light scattering of fractionated NaHA by MALS was performed in order to determine the MWD and molecular conformation. In these experiments, NaHA molecules exhibited an extended structure from a formerly rather compact geometry when the dissolving temperature was raised to 90 °C. This study also showed a clear difference in the MWD of NaHA when a preliminary filtration process was applied.

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Effect of dissolution temperature on the structures of sodium hyaluronate by flow field-flow fractionation/multiangle light scattering

Abstract

Bibliographical note

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