Effect of cardiopulmonary resuscitation on restoration of myocardial ATP in prolonged ventricular fibrillation

Han Joo Choi, Tuyet Nguyen, Kyu Sang Park, Kyoung Chul Cha, Hyun Kim, Kang Hyun Lee, Sung Oh Hwang

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Background: There has been controversy over whether a short period of cardiopulmonary resuscitation (CPR) prior to defibrillation improves survival in patients who experienced a sudden cardiac arrest. However, there have been no reports about whether CPR restores the myocardial energy source during prolonged ventricular fibrillation (VF). The aim of this study is to investigate the effect of CPR in restoring myocardial high energy phosphates during prolonged VF. Methods and results: Seventy-two adult male Sprague-Dawley rats were used in this study. Baseline adenosine triphosphate (ATP) and adenosine diphosphate (ADP) prior to induction of VF were measured in nine rats, the No-VF group. Sixty-three rats were subjected to 4. min of untreated VF. Animals were then randomized into two groups: No-CPR (n=37) and CPR (n=26). In the No-CPR group, ATPs and ADPs were measured at 4. min (No-CPR4), 6. min (No-CPR6), 8. min (No-CPR8) or 10. min (No-CPR10) after the induction of VF. The CPR group received 2. min (CPR2), 4. min (CPR4) or 6. min (CPR6) of mechanical chest compressions before ATP was measured.Myocardial ATP (nmol/mg protein) was decreased as VF duration was prolonged (No-VF: 5.49±1.71, No-CPR4: 4.27±1.58, No-CPR6: 4.13±1.31, No-CPR8: 3.77±1.42, No-CPR10: 3.52±0.90, p<0.05 between each of No-CPRs vs. No-VF). Two minutes of CPR restored myocardial ATP to the level of No-VF group (5.27±1.67. nmol/mg protein in CPR2, p>0.05 vs. No-VF group). However, myocardial ATP (nmol/mg protein) decreased if the duration of CPR was longer than 2. min (CPR4: 3.77±1.05, CPR6: 3.49±1.08, p<0.05 between CPR4 and CPR6 vs. No-VF). Conclusions: CPR for 2. min helps to maintain myocardial ATP after prolonged VF.

Original languageEnglish
Pages (from-to)108-113
Number of pages6
Issue number1
Publication statusPublished - 2013 Jan

Bibliographical note

Funding Information:
This work was supported by the Research Program (2009) of Wonju College of Medicine, Yonsei University, Republic of Korea .

All Science Journal Classification (ASJC) codes

  • Emergency Medicine
  • Emergency
  • Cardiology and Cardiovascular Medicine


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