Down syndrome critical region 2 protein inhibits the transcriptional activity of peroxisome proliferator-activated receptor β in HEK293 cells

Hae Jin Song; Joongkyu Park; Su Ryeon Seo; Jongsun Kim; Seung R. Paik; Kwang Chul Chung

doi:10.1016/j.bbrc.2008.09.017

Down syndrome critical region 2 protein inhibits the transcriptional activity of peroxisome proliferator-activated receptor β in HEK293 cells

Hae Jin Song, Joongkyu Park, Su Ryeon Seo, Jongsun Kim, Seung R. Paik, Kwang Chul Chung

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Down syndrome is mainly caused by a trisomy of chromosome 21. The Down syndrome critical region 2 (DSCR2) gene is located within a part of chromosome 21, the Down syndrome critical region (DSCR). To investigate the function of DSCR2, we sought to identify DSCR2-interacting proteins using yeast two-hybrid assays. A human fetal brain cDNA library was screened, and DSCR2 was found to interact with a member of the nuclear receptor superfamily, peroxisome proliferator-activated receptor β, (PPARβ). A co-immunoprecipitation assay demonstrated that DSCR2 physically interacts with PPARβ in mammalian HEK293 cells. DSCR2 also inhibited the ligand-induced transcriptional activity of PPARβ. Furthermore, PPARβ also decreased the solubility of DSCR2, which increased levels of insoluble DSCR2.

Original language	English
Pages (from-to)	478-482
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	376
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2008.09.017
Publication status	Published - 2008 Nov 21

Bibliographical note

Funding Information:
We thank F. Henrique-Silvia, R.M. Evans, and J.P. Vanden-Heuvel for providing plasmids. This work was supported by grants from the Brain Research Center of the 21st Century Frontier Research Program Technology (M103KV010011-06K2201-01110 to K.C.C.) and from the Korea Science and Engineering Foundation (KOSEF) through National Research Laboratory Program (R04-2007-000-20014-0 to K.C.C.) funded by the Ministry of Science, Republic of Korea. This work was also partly supported by KOSEF grants (R11-2007-040-01005-0 and R01-2007-000-20089-0 to K.C.C.), a grant from the Korea Health 21 R&D Project (A080551 and A060440 to K.C.C.), Ministry of Health & Welfare, and by the Korea Research Foundation Grant (KRF-2004-005-E0017 to K.C.C.).

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2008.09.017

Cite this

@article{ba121c64f079486da99976889aa429a8,

title = "Down syndrome critical region 2 protein inhibits the transcriptional activity of peroxisome proliferator-activated receptor β in HEK293 cells",

abstract = "Down syndrome is mainly caused by a trisomy of chromosome 21. The Down syndrome critical region 2 (DSCR2) gene is located within a part of chromosome 21, the Down syndrome critical region (DSCR). To investigate the function of DSCR2, we sought to identify DSCR2-interacting proteins using yeast two-hybrid assays. A human fetal brain cDNA library was screened, and DSCR2 was found to interact with a member of the nuclear receptor superfamily, peroxisome proliferator-activated receptor β, (PPARβ). A co-immunoprecipitation assay demonstrated that DSCR2 physically interacts with PPARβ in mammalian HEK293 cells. DSCR2 also inhibited the ligand-induced transcriptional activity of PPARβ. Furthermore, PPARβ also decreased the solubility of DSCR2, which increased levels of insoluble DSCR2.",

author = "Song, {Hae Jin} and Joongkyu Park and Seo, {Su Ryeon} and Jongsun Kim and Paik, {Seung R.} and Chung, {Kwang Chul}",

note = "Funding Information: We thank F. Henrique-Silvia, R.M. Evans, and J.P. Vanden-Heuvel for providing plasmids. This work was supported by grants from the Brain Research Center of the 21st Century Frontier Research Program Technology (M103KV010011-06K2201-01110 to K.C.C.) and from the Korea Science and Engineering Foundation (KOSEF) through National Research Laboratory Program (R04-2007-000-20014-0 to K.C.C.) funded by the Ministry of Science, Republic of Korea. This work was also partly supported by KOSEF grants (R11-2007-040-01005-0 and R01-2007-000-20089-0 to K.C.C.), a grant from the Korea Health 21 R&D Project (A080551 and A060440 to K.C.C.), Ministry of Health & Welfare, and by the Korea Research Foundation Grant (KRF-2004-005-E0017 to K.C.C.).",

year = "2008",

month = nov,

day = "21",

doi = "10.1016/j.bbrc.2008.09.017",

language = "English",

volume = "376",

pages = "478--482",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - Down syndrome critical region 2 protein inhibits the transcriptional activity of peroxisome proliferator-activated receptor β in HEK293 cells

AU - Song, Hae Jin

AU - Park, Joongkyu

AU - Seo, Su Ryeon

AU - Kim, Jongsun

AU - Paik, Seung R.

AU - Chung, Kwang Chul

N1 - Funding Information: We thank F. Henrique-Silvia, R.M. Evans, and J.P. Vanden-Heuvel for providing plasmids. This work was supported by grants from the Brain Research Center of the 21st Century Frontier Research Program Technology (M103KV010011-06K2201-01110 to K.C.C.) and from the Korea Science and Engineering Foundation (KOSEF) through National Research Laboratory Program (R04-2007-000-20014-0 to K.C.C.) funded by the Ministry of Science, Republic of Korea. This work was also partly supported by KOSEF grants (R11-2007-040-01005-0 and R01-2007-000-20089-0 to K.C.C.), a grant from the Korea Health 21 R&D Project (A080551 and A060440 to K.C.C.), Ministry of Health & Welfare, and by the Korea Research Foundation Grant (KRF-2004-005-E0017 to K.C.C.).

PY - 2008/11/21

Y1 - 2008/11/21

N2 - Down syndrome is mainly caused by a trisomy of chromosome 21. The Down syndrome critical region 2 (DSCR2) gene is located within a part of chromosome 21, the Down syndrome critical region (DSCR). To investigate the function of DSCR2, we sought to identify DSCR2-interacting proteins using yeast two-hybrid assays. A human fetal brain cDNA library was screened, and DSCR2 was found to interact with a member of the nuclear receptor superfamily, peroxisome proliferator-activated receptor β, (PPARβ). A co-immunoprecipitation assay demonstrated that DSCR2 physically interacts with PPARβ in mammalian HEK293 cells. DSCR2 also inhibited the ligand-induced transcriptional activity of PPARβ. Furthermore, PPARβ also decreased the solubility of DSCR2, which increased levels of insoluble DSCR2.

AB - Down syndrome is mainly caused by a trisomy of chromosome 21. The Down syndrome critical region 2 (DSCR2) gene is located within a part of chromosome 21, the Down syndrome critical region (DSCR). To investigate the function of DSCR2, we sought to identify DSCR2-interacting proteins using yeast two-hybrid assays. A human fetal brain cDNA library was screened, and DSCR2 was found to interact with a member of the nuclear receptor superfamily, peroxisome proliferator-activated receptor β, (PPARβ). A co-immunoprecipitation assay demonstrated that DSCR2 physically interacts with PPARβ in mammalian HEK293 cells. DSCR2 also inhibited the ligand-induced transcriptional activity of PPARβ. Furthermore, PPARβ also decreased the solubility of DSCR2, which increased levels of insoluble DSCR2.

UR - http://www.scopus.com/inward/record.url?scp=53149147376&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=53149147376&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2008.09.017

DO - 10.1016/j.bbrc.2008.09.017

M3 - Article

C2 - 18793612

AN - SCOPUS:53149147376

SN - 0006-291X

VL - 376

SP - 478

EP - 482

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Down syndrome critical region 2 protein inhibits the transcriptional activity of peroxisome proliferator-activated receptor β in HEK293 cells

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this