Down-regulation of phospholipase D during differentiation of mouse F9 teratocarcinoma cells

Do Sik Min; Kyung Sun Shin; Eung Guk Kim; Seung Ryul Kim; Shin Hee Yoon; Myung Suk Kim; Yang Hyeok Jo

doi:10.1016/S0014-5793(99)00798-X

Down-regulation of phospholipase D during differentiation of mouse F9 teratocarcinoma cells

Do Sik Min, Kyung Sun Shin, Eung Guk Kim, Seung Ryul Kim, Shin Hee Yoon, Myung Suk Kim, Yang Hyeok Jo

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Phospholipase D has been recognized as playing an important role in signal transduction in many types of cells. We investigated the expression of phospholipase D during the differentiation of F9 embryonal teratocarcinoma cells. The ADP ribosylation factor-dependent phospholipase D activity, as measured by an in vitro assay, and H₂O₂-induced phospholipase D activity and phospholipase D protein content in whole cells were decreased during the differentiation of F9 cells induced by a combination of dibutyryl cyclic AMP and all-trans retinoic acid. In contrast, these changes were not observed when cells were induced by retinoic acid. These results suggest that down-regulation of phospholipase D protein is associated with differentiation of F9 cells to a parietal endoderm lineage. Copyright (C) 1999 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	197-200
Number of pages	4
Journal	FEBS Letters
Volume	454
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(99)00798-X
Publication status	Published - 1999 Jul 9

Bibliographical note

Funding Information:
A~knowledgements." This work was supported by a research Grant (HMP-98-N-1-0012) from the Good Health R and D Project and the Ministry of Health and Welfare, R.O.K.

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(99)00798-X

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title = "Down-regulation of phospholipase D during differentiation of mouse F9 teratocarcinoma cells",

abstract = "Phospholipase D has been recognized as playing an important role in signal transduction in many types of cells. We investigated the expression of phospholipase D during the differentiation of F9 embryonal teratocarcinoma cells. The ADP ribosylation factor-dependent phospholipase D activity, as measured by an in vitro assay, and H2O2-induced phospholipase D activity and phospholipase D protein content in whole cells were decreased during the differentiation of F9 cells induced by a combination of dibutyryl cyclic AMP and all-trans retinoic acid. In contrast, these changes were not observed when cells were induced by retinoic acid. These results suggest that down-regulation of phospholipase D protein is associated with differentiation of F9 cells to a parietal endoderm lineage. Copyright (C) 1999 Federation of European Biochemical Societies.",

author = "Min, {Do Sik} and Shin, {Kyung Sun} and Kim, {Eung Guk} and Kim, {Seung Ryul} and Yoon, {Shin Hee} and Kim, {Myung Suk} and Jo, {Yang Hyeok}",

note = "Funding Information: A~knowledgements.{"} This work was supported by a research Grant (HMP-98-N-1-0012) from the Good Health R and D Project and the Ministry of Health and Welfare, R.O.K.",

year = "1999",

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doi = "10.1016/S0014-5793(99)00798-X",

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T1 - Down-regulation of phospholipase D during differentiation of mouse F9 teratocarcinoma cells

AU - Min, Do Sik

AU - Shin, Kyung Sun

AU - Kim, Eung Guk

AU - Kim, Seung Ryul

AU - Yoon, Shin Hee

AU - Kim, Myung Suk

AU - Jo, Yang Hyeok

N1 - Funding Information: A~knowledgements." This work was supported by a research Grant (HMP-98-N-1-0012) from the Good Health R and D Project and the Ministry of Health and Welfare, R.O.K.

PY - 1999/7/9

Y1 - 1999/7/9

N2 - Phospholipase D has been recognized as playing an important role in signal transduction in many types of cells. We investigated the expression of phospholipase D during the differentiation of F9 embryonal teratocarcinoma cells. The ADP ribosylation factor-dependent phospholipase D activity, as measured by an in vitro assay, and H2O2-induced phospholipase D activity and phospholipase D protein content in whole cells were decreased during the differentiation of F9 cells induced by a combination of dibutyryl cyclic AMP and all-trans retinoic acid. In contrast, these changes were not observed when cells were induced by retinoic acid. These results suggest that down-regulation of phospholipase D protein is associated with differentiation of F9 cells to a parietal endoderm lineage. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - Phospholipase D has been recognized as playing an important role in signal transduction in many types of cells. We investigated the expression of phospholipase D during the differentiation of F9 embryonal teratocarcinoma cells. The ADP ribosylation factor-dependent phospholipase D activity, as measured by an in vitro assay, and H2O2-induced phospholipase D activity and phospholipase D protein content in whole cells were decreased during the differentiation of F9 cells induced by a combination of dibutyryl cyclic AMP and all-trans retinoic acid. In contrast, these changes were not observed when cells were induced by retinoic acid. These results suggest that down-regulation of phospholipase D protein is associated with differentiation of F9 cells to a parietal endoderm lineage. Copyright (C) 1999 Federation of European Biochemical Societies.

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JO - FEBS Letters

JF - FEBS Letters

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Down-regulation of phospholipase D during differentiation of mouse F9 teratocarcinoma cells

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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