TY - JOUR
T1 - DNA/RNA chimera templates improve the emission intensity and target the accessibility of silver nanocluster-based sensors for human microRNA detection
AU - Shah, Pratik
AU - Choi, Suk Won
AU - Kim, Ho Jin
AU - Cho, Seok Keun
AU - Thulstrup, Peter Waaben
AU - Bjerrum, Morten Jannik
AU - Bhang, Yong Joo
AU - Ahn, Jong Cheol
AU - Yang, Seong Wook
N1 - Publisher Copyright:
© 2015 The Royal Society of Chemistry.
PY - 2015/5/21
Y1 - 2015/5/21
N2 - In recent years microRNAs (miRNAs) have been established as important biomarkers in a variety of diseases including cancer, diabetes, cardiovascular disease, aging, Alzheimer's disease, asthma, autoimmune disease and liver diseases. As a consequence, a variety of monitoring methods for miRNAs have been developed, including a fast and simple method for miRNA detection by exploitation of the unique photoluminescence of DNA-templated silver nanoclusters (DNA/AgNCs). To increase the versatility of the AgNC-based method, we have adopted DNA/RNA chimera templates for AgNC-based probes, allowing response from several human miRNAs which are hardly detectable with DNA-based probes. Here, we demonstrate in detail the power of DNA/RNA chimera/AgNC probes in detecting two human miRNAs, let-7a and miR-200c. The DNA/RNA chimera-based probes are highly efficient to determine the level of miRNAs in several human cell lines.
AB - In recent years microRNAs (miRNAs) have been established as important biomarkers in a variety of diseases including cancer, diabetes, cardiovascular disease, aging, Alzheimer's disease, asthma, autoimmune disease and liver diseases. As a consequence, a variety of monitoring methods for miRNAs have been developed, including a fast and simple method for miRNA detection by exploitation of the unique photoluminescence of DNA-templated silver nanoclusters (DNA/AgNCs). To increase the versatility of the AgNC-based method, we have adopted DNA/RNA chimera templates for AgNC-based probes, allowing response from several human miRNAs which are hardly detectable with DNA-based probes. Here, we demonstrate in detail the power of DNA/RNA chimera/AgNC probes in detecting two human miRNAs, let-7a and miR-200c. The DNA/RNA chimera-based probes are highly efficient to determine the level of miRNAs in several human cell lines.
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U2 - 10.1039/c5an00093a
DO - 10.1039/c5an00093a
M3 - Article
C2 - 25759134
AN - SCOPUS:84928978263
SN - 0003-2654
VL - 140
SP - 3422
EP - 3430
JO - Analyst
JF - Analyst
IS - 10
ER -
