DNA microarray-based detection of nosocomial pathogenic Pseudomonas aeruginosa and Acinetobacter baumannii

Ki Chang Keum; Seung Min Yoo; Sang Yup Lee; Kyung Hee Chang; Nae Choon Yoo; Won Min Yoo; June Myung Kim; Jun Yong Choi; Jong Seok Kim; Gene Lee

doi:10.1016/j.mcp.2005.09.001

DNA microarray-based detection of nosocomial pathogenic Pseudomonas aeruginosa and Acinetobacter baumannii

Ki Chang Keum, Seung Min Yoo, Sang Yup Lee, Kyung Hee Chang, Nae Choon Yoo, Won Min Yoo, June Myung Kim, Jun Yong Choi, Jong Seok Kim, Gene Lee

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Infection by nosocomial pathogenic bacteria is increasingly becoming a major threat to the patients in the hospital. We have developed a diagnostic DNA microarray for the detection of two important nosocomial pathogens, Pseudomonas aeruginosa and Acinetobacter baumannii. The diagnostic DNA microarray contains the species-specific probes of 15mer oligonucleotides designed based on the sequences of 23S ribosomal DNA. The performance of DNA microarray in diagnosing P. aeruginosa and A. baumannii was evaluated using reference bacteria as well as clinical specimens such as blood, stool, pus, sputum, urine and cerebrospinal fluid. Using this DNA microarray, A. baumannii could be successfully detected in 11 out of 13 clinical specimens, thus giving the sensitivity of 84.6% with the specificity of 100% and the positive predictive value of 100%. P. aeruginosa could also be detected in 25 out of 26 clinical specimens, showing the sensitivity of 96.2%, the specificity of 100%, and the positive predictive value of 100%. These results suggest that two nosocomial pathogens, P. aeruginosa and A. baumannii, can be efficiently diagnosed by using the DNA microarray developed in this study.

Original language	English
Pages (from-to)	42-50
Number of pages	9
Journal	Molecular and Cellular Probes
Volume	20
Issue number	1
DOIs	https://doi.org/10.1016/j.mcp.2005.09.001
Publication status	Published - 2006 Feb

Bibliographical note

Funding Information:
The work was supported by Medigenes Co. and Korean Systems Biology Research Program (M10309020000-03B5002-00000) of the Ministry of Science and Technology. Further supports by the LG Chem Chair Professorship, KOSEF through the CUPS, IBM-SUR program and BK21 program are appreciated.

All Science Journal Classification (ASJC) codes

Molecular Biology
Cell Biology

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10.1016/j.mcp.2005.09.001

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title = "DNA microarray-based detection of nosocomial pathogenic Pseudomonas aeruginosa and Acinetobacter baumannii",

abstract = "Infection by nosocomial pathogenic bacteria is increasingly becoming a major threat to the patients in the hospital. We have developed a diagnostic DNA microarray for the detection of two important nosocomial pathogens, Pseudomonas aeruginosa and Acinetobacter baumannii. The diagnostic DNA microarray contains the species-specific probes of 15mer oligonucleotides designed based on the sequences of 23S ribosomal DNA. The performance of DNA microarray in diagnosing P. aeruginosa and A. baumannii was evaluated using reference bacteria as well as clinical specimens such as blood, stool, pus, sputum, urine and cerebrospinal fluid. Using this DNA microarray, A. baumannii could be successfully detected in 11 out of 13 clinical specimens, thus giving the sensitivity of 84.6% with the specificity of 100% and the positive predictive value of 100%. P. aeruginosa could also be detected in 25 out of 26 clinical specimens, showing the sensitivity of 96.2%, the specificity of 100%, and the positive predictive value of 100%. These results suggest that two nosocomial pathogens, P. aeruginosa and A. baumannii, can be efficiently diagnosed by using the DNA microarray developed in this study.",

author = "Keum, {Ki Chang} and Yoo, {Seung Min} and Lee, {Sang Yup} and Chang, {Kyung Hee} and Yoo, {Nae Choon} and Yoo, {Won Min} and Kim, {June Myung} and Choi, {Jun Yong} and Kim, {Jong Seok} and Gene Lee",

note = "Funding Information: The work was supported by Medigenes Co. and Korean Systems Biology Research Program (M10309020000-03B5002-00000) of the Ministry of Science and Technology. Further supports by the LG Chem Chair Professorship, KOSEF through the CUPS, IBM-SUR program and BK21 program are appreciated.",

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AU - Yoo, Won Min

AU - Kim, June Myung

AU - Choi, Jun Yong

AU - Kim, Jong Seok

AU - Lee, Gene

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N2 - Infection by nosocomial pathogenic bacteria is increasingly becoming a major threat to the patients in the hospital. We have developed a diagnostic DNA microarray for the detection of two important nosocomial pathogens, Pseudomonas aeruginosa and Acinetobacter baumannii. The diagnostic DNA microarray contains the species-specific probes of 15mer oligonucleotides designed based on the sequences of 23S ribosomal DNA. The performance of DNA microarray in diagnosing P. aeruginosa and A. baumannii was evaluated using reference bacteria as well as clinical specimens such as blood, stool, pus, sputum, urine and cerebrospinal fluid. Using this DNA microarray, A. baumannii could be successfully detected in 11 out of 13 clinical specimens, thus giving the sensitivity of 84.6% with the specificity of 100% and the positive predictive value of 100%. P. aeruginosa could also be detected in 25 out of 26 clinical specimens, showing the sensitivity of 96.2%, the specificity of 100%, and the positive predictive value of 100%. These results suggest that two nosocomial pathogens, P. aeruginosa and A. baumannii, can be efficiently diagnosed by using the DNA microarray developed in this study.

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DNA microarray-based detection of nosocomial pathogenic Pseudomonas aeruginosa and Acinetobacter baumannii

Abstract

Bibliographical note

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