Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries

Moon Jung Kim, Il Kwon Bae, Seok Hoon Jeong, So Hyun Kim, Jae Hoon Song, Jae Young Choi, Sang Sun Yoon, Visanu Thamlikitkul, Po Ren Hsueh, Rohani Md Yasin, M. K. Lalitha, Kyungwon Lee

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)


Objectives: To investigate the epidemiological traits of metallo-b-lactamase (MBL)-producing Pseudomonas aeruginosa (MPPA) clinical isolates collected by the Asian Network for Surveillance of Resistant Pathogens (ANSORP). Methods:Atotal of16MPPAclinical isolateswerecollected fromsix Asian countries in2000to2009byANSORP.The MBLgenewasdetected byPCRamplification. The genetic organization of the class1integroncarrying theMBLgene cassettewas investigated by PCR mapping and sequencing. Southern blotting, repetitive sequence-based PCR and multilocus sequence typing (MLST) experiments were performed to characterize the isolates. Results: PCR and sequencing experiments detected the blaVIM-2 (n=12), blaVIM-3 (n=1), blaIMP-6 (n=2) and blaIMP-26 (n=1) genes. The MBL genes were located on the chromosome in all isolates except one. Furthermore, all the MBL genes were located in a class 1 integron. All the MPPA isolates from Malaysia, Thailand, Sri Lanka and Korea were identified as sequence type (ST) 235 by MLST. Three VIM-2-producing isolates fromIndia were identified as ST773, and one isolate harbouring VIM-3 from Taiwan was identified as ST298. Conclusions: P. aeruginosa ST235might play a role in dissemination of MBL genes in Asian countries.

Original languageEnglish
Article numberdkt269
Pages (from-to)2820-2824
Number of pages5
JournalJournal of Antimicrobial Chemotherapy
Issue number12
Publication statusPublished - 2013 Dec

Bibliographical note

Funding Information:
This study was supported by a grant from the Korea Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (A110096).

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)


Dive into the research topics of 'Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries'. Together they form a unique fingerprint.

Cite this