Disordered plasmonic substrate-based wide-field super-resolution imaging

Hongki Lee, Hajun Yoo, Woo Joong Rhee, Gwiyeong Moon, Changhun Lee, Jeon Soo Shin, Donghyun Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution


We have investigated the feasibility of disordered plasmonic nanocomposites for super-resolution imaging. Annealing-based nanocomposite substrate has a great potential in biomedical optical and sensing technology because it can be mass-produced without difficult manufacturing processes. We introduce a new approach for wide-field super-resolution fluorescence imaging based on the nanocomposite island substrates, which we call nanospeckle illumination microscopy (NanoSIM). Near-field speckle patterns produced on disordered nanoisland substrates can help reconstruction of high-resolution fluorescence images with appropriate basis images. We have acquired basis images using azimuthal scanning illumination (ASI). Each ASI produces nonuniform nanoscale near-field speckles which can excite fluorescent dyes within a subdiffraction-limited area. While exploiting the random nature of plasmonic nanocomposite, NanoSIM does not require any specific polarization state to be maintained for ASI. We have tested NanoSIM to obtain super-resolved mages of molecules on the HeLa cell membrane. The full-width-at-half maximum was shown to improve by more than three times over the diffraction-limit with 360 basis images. Reconstructed images of gangliosides distribution on the HeLa cell suggest that fewer basis images may produce almost the same resolution with a shorter computation time. The optical resolution and sensitivity of disordered plasmonic substrate can be further enhanced by controlling the geometrical features of nanoislands structure.

Original languageEnglish
Title of host publicationPlasmonics in Biology and Medicine XIX
EditorsTuan Vo-Dinh, Ho-Pui A. Ho, Krishanu Ray
ISBN (Electronic)9781510648272
Publication statusPublished - 2022
EventPlasmonics in Biology and Medicine XIX 2022 - Virtual, Online
Duration: 2022 Feb 202022 Feb 24

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferencePlasmonics in Biology and Medicine XIX 2022
CityVirtual, Online

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation grant funded by the Korean government (2019R1A4A1025958) and the Korea Medical Device Development Fund (Project Number: KMDF_PR_20200901_0088 and KMDF_PR_20200901_0103). W.J.R. and J.-S.S. acknowledge the support by the NRF (2019R1A6A1A03032869).

Publisher Copyright:
© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


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