Sample processing, especially that involving nucleic acid extraction, is a prerequisite step for the isolation of high quantities of relatively pure DNA for downstream analyses in many life science and biomedical engineering studies. However, existing methods still have major problems, including labor-intensive time-consuming methods and high costs, as well as requirements for a centrifuge and the complex fabrication of filters and membranes. Here, we first report a versatile Dimethyl adipimidate/Thin film based Sample processing (DTS) procedure without the limitations of existing methods. This procedure is useful for the extraction of DNA from a variety of sources, including 6 eukaryotic cells, 6 bacteria cells, and 2 body fluids in a single step. Specifically, the DTS procedure does not require a centrifuge and has improved time efficiency (30 min), affordability, and sensitivity in downstream analysis. We validated the DTS procedure for the extraction of DNA from human body fluids, as well as confirmed that the quality and quantity of the extracted DNA were sufficient to allow robust detection of genetic and epigenetic biomarkers in downstream analysis.
|Publication status||Published - 2015 Sept 15|
Bibliographical noteFunding Information:
We would like to thank Dr. Timothy Barkham (Tan Tock Sheng Hospital, Singapore) for providing the mycobacterium abscessus and gordonae and also thank Dr. Hyun-Gyun Yuk (Department of Chemistry, National University of Singapore, Singapore) for providing the salmonella strain. Additionally, we would like to thank Jaeyun Yoon for her technical support. This work was supported by the Agency for Science, Technology and Research (A*STAR) Biomedical Engineering Program Grant (1421480025), Singapore.
© 2015, Nature Publishing Group. All rights reserved.
All Science Journal Classification (ASJC) codes