Dihydroxyacetone synthase from a methanol-utilizing carboxydobacterium, Acinetobacter sp. strain JC1 DSM 3803

Young T. Ro, Chi Y. Eom, Taeksun Song, Jin W. Cho, Young M. Kim

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


Acinetobacter sp. strain JC1 DSM 3803, a carboxydobacterium, grown on methanol was found to show dihydroxyacetone synthase, dihydroxyacetone kinase, and ribulose 1,5-bisphosphate carboxylase, but no hydroxypyruvate reductase and very low hexulose 6-phosphate synthase, activities. The dihydroxyacetone synthase was found to be expressed earlier than the ribulose 1,5-bisphosphate carboxylase. The dihydroxyacetone synthase was purified 19- fold in eight steps to homogeneity, with a yield of 9%. The final specific activity of the purified enzyme was 1.12 μmol of NADH oxidized per min per mg of protein. The molecular weight of the native enzyme was determined to be 140,000. Sodium dodecyl sulfate-gel electrophoresis revealed a subunit of molecular weight 73,000. The optimum temperature and pH were 30°C and 7.0, respectively. The enzyme was inactivated very rapidly at 70°C. The enzyme required Mg2+ and thiamine pyrophosphate for maximal activity. Xylulose 5- phosphate was found to be the best substrate when formaldehyde was used as a glycolaldehyde acceptor. Erythrose 4-phosphate, glycolaldehyde, and formaldehyde were found to act as excellent substrates when xylulose 5- phosphate was used as a glycolaldehyde donor. The K(m)s for formaldehyde and xylulose 5-phosphate were 1.86 mM and 33.3 μM, respectively. The enzyme produced dihydroxyacetone from formaldehyde and xylulose 5-phosphate. The enzyme was found to be expressed only in cells grown on methanol and shared no immunological properties with the yeast dihydroxyacetone synthase.

Original languageEnglish
Pages (from-to)6041-6047
Number of pages7
JournalJournal of Bacteriology
Issue number19
Publication statusPublished - 1997 Oct

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology


Dive into the research topics of 'Dihydroxyacetone synthase from a methanol-utilizing carboxydobacterium, Acinetobacter sp. strain JC1 DSM 3803'. Together they form a unique fingerprint.

Cite this