Differential expression of phospholipases D1 and D2 in mouse tissues

Heechul Kim; Jeeyoung Lee; Seungjoon Kim; Min Kyoung Shin; Do Sik Min; Taekyun Shin

doi:10.1016/j.cellbi.2006.09.020

Differential expression of phospholipases D1 and D2 in mouse tissues

Heechul Kim, Jeeyoung Lee, Seungjoon Kim, Min Kyoung Shin, Do Sik Min, Taekyun Shin

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

The differential expression of phospholipase D (PLD) isozymes, which include PLD1 and PLD2, was examined in various murine tissues, including the cerebrum, cerebellum, heart, lung, liver, spleen, stomach, pancreas, ileum, colon, adrenal gland, kidneys, testes, ovaries, and uterus. In Western blot analysis, only PLD1 was detected in the heart and ovary, while only PLD2 was detected in the pancreas and ileum. Both PLD1 and PLD2 were strongly expressed in the cerebrum, cerebellum, and lung, and both were also expressed in the liver, spleen, stomach, colon, kidney, testes, and uterus. Immunohistochemistry showed intense PLD immunostaining in the cerebrum, cerebellum, lungs, intestines, and testis, and weak PLD immunostaining in the liver, kidneys, spleen, and heart. These findings suggest that PLD1 and PLD2 are differentially expressed in the various organs of mice, and that each PLD isozyme plays a distinct role in each organ.

Original language	English
Pages (from-to)	148-155
Number of pages	8
Journal	Cell Biology International
Volume	31
Issue number	2
DOIs	https://doi.org/10.1016/j.cellbi.2006.09.020
Publication status	Published - 2007 Feb

Bibliographical note

Funding Information:
This work was supported by a program of the Basic Atomic Energy Research Institute (BAERI), which is a part of the Nuclear R & D Programs funded by the Ministry of Science & Technology (MOST) of Korea. This work was supported by Pusan National University Research Grant, 2004.

All Science Journal Classification (ASJC) codes

Cell Biology

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10.1016/j.cellbi.2006.09.020

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title = "Differential expression of phospholipases D1 and D2 in mouse tissues",

abstract = "The differential expression of phospholipase D (PLD) isozymes, which include PLD1 and PLD2, was examined in various murine tissues, including the cerebrum, cerebellum, heart, lung, liver, spleen, stomach, pancreas, ileum, colon, adrenal gland, kidneys, testes, ovaries, and uterus. In Western blot analysis, only PLD1 was detected in the heart and ovary, while only PLD2 was detected in the pancreas and ileum. Both PLD1 and PLD2 were strongly expressed in the cerebrum, cerebellum, and lung, and both were also expressed in the liver, spleen, stomach, colon, kidney, testes, and uterus. Immunohistochemistry showed intense PLD immunostaining in the cerebrum, cerebellum, lungs, intestines, and testis, and weak PLD immunostaining in the liver, kidneys, spleen, and heart. These findings suggest that PLD1 and PLD2 are differentially expressed in the various organs of mice, and that each PLD isozyme plays a distinct role in each organ.",

author = "Heechul Kim and Jeeyoung Lee and Seungjoon Kim and Shin, {Min Kyoung} and Min, {Do Sik} and Taekyun Shin",

note = "Funding Information: This work was supported by a program of the Basic Atomic Energy Research Institute (BAERI), which is a part of the Nuclear R & D Programs funded by the Ministry of Science & Technology (MOST) of Korea. This work was supported by Pusan National University Research Grant, 2004.",

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AU - Kim, Seungjoon

AU - Shin, Min Kyoung

AU - Min, Do Sik

AU - Shin, Taekyun

N1 - Funding Information: This work was supported by a program of the Basic Atomic Energy Research Institute (BAERI), which is a part of the Nuclear R & D Programs funded by the Ministry of Science & Technology (MOST) of Korea. This work was supported by Pusan National University Research Grant, 2004.

PY - 2007/2

Y1 - 2007/2

N2 - The differential expression of phospholipase D (PLD) isozymes, which include PLD1 and PLD2, was examined in various murine tissues, including the cerebrum, cerebellum, heart, lung, liver, spleen, stomach, pancreas, ileum, colon, adrenal gland, kidneys, testes, ovaries, and uterus. In Western blot analysis, only PLD1 was detected in the heart and ovary, while only PLD2 was detected in the pancreas and ileum. Both PLD1 and PLD2 were strongly expressed in the cerebrum, cerebellum, and lung, and both were also expressed in the liver, spleen, stomach, colon, kidney, testes, and uterus. Immunohistochemistry showed intense PLD immunostaining in the cerebrum, cerebellum, lungs, intestines, and testis, and weak PLD immunostaining in the liver, kidneys, spleen, and heart. These findings suggest that PLD1 and PLD2 are differentially expressed in the various organs of mice, and that each PLD isozyme plays a distinct role in each organ.

AB - The differential expression of phospholipase D (PLD) isozymes, which include PLD1 and PLD2, was examined in various murine tissues, including the cerebrum, cerebellum, heart, lung, liver, spleen, stomach, pancreas, ileum, colon, adrenal gland, kidneys, testes, ovaries, and uterus. In Western blot analysis, only PLD1 was detected in the heart and ovary, while only PLD2 was detected in the pancreas and ileum. Both PLD1 and PLD2 were strongly expressed in the cerebrum, cerebellum, and lung, and both were also expressed in the liver, spleen, stomach, colon, kidney, testes, and uterus. Immunohistochemistry showed intense PLD immunostaining in the cerebrum, cerebellum, lungs, intestines, and testis, and weak PLD immunostaining in the liver, kidneys, spleen, and heart. These findings suggest that PLD1 and PLD2 are differentially expressed in the various organs of mice, and that each PLD isozyme plays a distinct role in each organ.

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Differential expression of phospholipases D1 and D2 in mouse tissues

Abstract

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