Differential expression of ADC mRNA during development and upon acid stress in soybean (Glycine max) hypocotyls

Kyoung Hee Nam, Sun Hi Lee, Joo Hun Lee

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42 Citations (Scopus)


Arginine decarboxylase (ADC) is one of the key enzymes in the biosynthesis of putrescine in plants. The regulation of its activity depends on the physiological condition, developmental stage, and type of tissue. We have cloned ADC cDNA from soybean (Glycine max) hypocotyls to understand the regulation mechanisms of this enzyme activity. Using the cDNA clone, we examined the relationship between changes in the ADC activity and the level of ADC mRNA during development, in different tissues, and upon acid stress. The ADC activity began to increase 2 d after initiation of germination, reached a peak at the 5th d, and then declined. This change in the enzyme activity was preceded by similar changes in the level of the mRNA. The ADC activity was expressed tissue-specifically; this expression was well correlated with the mRNA content of the respective tissues. Incubation of the 5-d-old hypocotyls in pH 3 potassium phosphate solution caused a rapid increase in ADC activity. Within 2 h of acid treatment, the ADC activity increased more than threefold. This increase was also preceded by a corresponding increase in the mRNA content and was also regulated tissue-specifically. These results suggest that the change in the content of ADC mRNA has an important role in the regulation of the enzyme activity during early development, in different tissues, and upon acid stress.

Original languageEnglish
Pages (from-to)1156-1166
Number of pages11
JournalPlant and Cell Physiology
Issue number10
Publication statusPublished - 1997 Oct

Bibliographical note

Funding Information:
We thank Dr. Malmberg for his generous gift of oat ADC cDNA which was used to screen the library at the initial stage of this experiment. We also thank Drs. Myeong Min Lee, Bin Gu Kang, Ky Young Park and Woo Taek Kim for their critical reading of the manuscript. This work was supported by a grant from KOSEF (91-05-00-05).

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology


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