Differential antigen processing by dendritic cell subsets in vivo

Diana Dudziak; Alice O. Kamphorst; Gordon F. Heidkamp; Veit R. Buchholz; Christine Trumpfheller; Sayuri Yamazaki; Cheolho Cheong; Kang Liu; Han Woong Lee; Gyu Park Chae; Ralph M. Steinman; Michel C. Nussenzweig

doi:10.1126/science.1136080

Differential antigen processing by dendritic cell subsets in vivo

Diana Dudziak, Alice O. Kamphorst, Gordon F. Heidkamp, Veit R. Buchholz, Christine Trumpfheller, Sayuri Yamazaki, Cheolho Cheong, Kang Liu, Han Woong Lee, Gyu Park Chae, Ralph M. Steinman, Michel C. Nussenzweig

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1114 Citations (Scopus)

Abstract

Dendritic cells (DCs) process and present self and foreign antigens to induce tolerance or immunity. In vitro models suggest that induction of immunity is controlled by regulating the presentation of antigen, but little is known about how DCs control antigen presentation in vivo. To examine antigen processing and presentation in vivo, we specifically targeted antigens to two major subsets of DCs by using chimeric monoclonal antibodies. Unlike CD8 ⁺ DCs that express the cell surface protein CD205, CD8^- DCs, which are positive for the 3301 antigen, are specialized for presentation on major histocompatibility complex (MHC) class II. This difference in antigen processing is intrinsic to the DC subsets and is associated with increased expression of proteins involved in MHC processing.

Original language	English
Pages (from-to)	107-111
Number of pages	5
Journal	Science
Volume	315
Issue number	5808
DOIs	https://doi.org/10.1126/science.1136080
Publication status	Published - 2007 Jan 5

All Science Journal Classification (ASJC) codes

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title = "Differential antigen processing by dendritic cell subsets in vivo",

abstract = "Dendritic cells (DCs) process and present self and foreign antigens to induce tolerance or immunity. In vitro models suggest that induction of immunity is controlled by regulating the presentation of antigen, but little is known about how DCs control antigen presentation in vivo. To examine antigen processing and presentation in vivo, we specifically targeted antigens to two major subsets of DCs by using chimeric monoclonal antibodies. Unlike CD8 + DCs that express the cell surface protein CD205, CD8- DCs, which are positive for the 3301 antigen, are specialized for presentation on major histocompatibility complex (MHC) class II. This difference in antigen processing is intrinsic to the DC subsets and is associated with increased expression of proteins involved in MHC processing.",

author = "Diana Dudziak and Kamphorst, {Alice O.} and Heidkamp, {Gordon F.} and Buchholz, {Veit R.} and Christine Trumpfheller and Sayuri Yamazaki and Cheolho Cheong and Kang Liu and Lee, {Han Woong} and Chae, {Gyu Park} and Steinman, {Ralph M.} and Nussenzweig, {Michel C.}",

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doi = "10.1126/science.1136080",

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AU - Dudziak, Diana

AU - Kamphorst, Alice O.

AU - Heidkamp, Gordon F.

AU - Buchholz, Veit R.

AU - Trumpfheller, Christine

AU - Yamazaki, Sayuri

AU - Cheong, Cheolho

AU - Liu, Kang

AU - Lee, Han Woong

AU - Chae, Gyu Park

AU - Steinman, Ralph M.

AU - Nussenzweig, Michel C.

PY - 2007/1/5

Y1 - 2007/1/5

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AB - Dendritic cells (DCs) process and present self and foreign antigens to induce tolerance or immunity. In vitro models suggest that induction of immunity is controlled by regulating the presentation of antigen, but little is known about how DCs control antigen presentation in vivo. To examine antigen processing and presentation in vivo, we specifically targeted antigens to two major subsets of DCs by using chimeric monoclonal antibodies. Unlike CD8 + DCs that express the cell surface protein CD205, CD8- DCs, which are positive for the 3301 antigen, are specialized for presentation on major histocompatibility complex (MHC) class II. This difference in antigen processing is intrinsic to the DC subsets and is associated with increased expression of proteins involved in MHC processing.

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Differential antigen processing by dendritic cell subsets in vivo

Abstract

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