Differences in doublecortin immunoreactivity and protein levels in the hippocampal dentate gyrus between adult and aged dogs

In Koo Hwang; Ki Yeon Yoo; Hua Li; Jung Hoon Choi; Young Guen Kwon; Youngkeun Ahn; In Se Lee; Moo Ho Won

doi:10.1007/s11064-007-9366-1

Differences in doublecortin immunoreactivity and protein levels in the hippocampal dentate gyrus between adult and aged dogs

In Koo Hwang, Ki Yeon Yoo, Hua Li, Jung Hoon Choi, Young Guen Kwon, Youngkeun Ahn, In Se Lee, Moo Ho Won

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39 Citations (Scopus)

Abstract

Doublecortin (DCX), a microtubule-associated protein, specifically expresses in neuronal precursors. This protein has been used as a marker for neuronal precursors and neurogenesis. In the present study, we observed differences in DCX immunoreactivity and its protein levels in the hippocampal dentate gyrus between adult and aged dogs. In the adult dog, DCX immunoreactive cells with well-stained processes were detected in the subgranular zone of the dentate gyrus. Numbers of DCX immunoreactive cells in the dentate gyrus of the aged dog were significantly decreased compared to those in the adult dog. DCX immunoreactive cells in both adult and aged dog did not show NeuN (a marker for mature neurons) immunoreactivity. NeuN immunoreactivity in the aged dog was poor compared to that in the adult dog. DCX protein level in the aged dentate gyrus was decreased by 80% compared to that in the adult dog. These results suggest that the reduction of DCX in the aged hippocampal dentate gyrus may be involved in some neural deficits related to the hippocampus.

Original language	English
Pages (from-to)	1604-1609
Number of pages	6
Journal	Neurochemical Research
Volume	32
Issue number	9
DOIs	https://doi.org/10.1007/s11064-007-9366-1
Publication status	Published - 2007 Sept

Bibliographical note

Funding Information:
Acknowledgements The authors would like to thank Mr. Seok Han, Mr. Seung Uk Lee and Ms. Hyun Sook Kim for their technical help in this study. This work was supported by the Nano/Bio program of Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (No. M10641450002-06N4145-00200).

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Biochemistry
Cellular and Molecular Neuroscience

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title = "Differences in doublecortin immunoreactivity and protein levels in the hippocampal dentate gyrus between adult and aged dogs",

abstract = "Doublecortin (DCX), a microtubule-associated protein, specifically expresses in neuronal precursors. This protein has been used as a marker for neuronal precursors and neurogenesis. In the present study, we observed differences in DCX immunoreactivity and its protein levels in the hippocampal dentate gyrus between adult and aged dogs. In the adult dog, DCX immunoreactive cells with well-stained processes were detected in the subgranular zone of the dentate gyrus. Numbers of DCX immunoreactive cells in the dentate gyrus of the aged dog were significantly decreased compared to those in the adult dog. DCX immunoreactive cells in both adult and aged dog did not show NeuN (a marker for mature neurons) immunoreactivity. NeuN immunoreactivity in the aged dog was poor compared to that in the adult dog. DCX protein level in the aged dentate gyrus was decreased by 80% compared to that in the adult dog. These results suggest that the reduction of DCX in the aged hippocampal dentate gyrus may be involved in some neural deficits related to the hippocampus.",

author = "Hwang, {In Koo} and Yoo, {Ki Yeon} and Hua Li and Choi, {Jung Hoon} and Kwon, {Young Guen} and Youngkeun Ahn and Lee, {In Se} and Won, {Moo Ho}",

note = "Funding Information: Acknowledgements The authors would like to thank Mr. Seok Han, Mr. Seung Uk Lee and Ms. Hyun Sook Kim for their technical help in this study. This work was supported by the Nano/Bio program of Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (No. M10641450002-06N4145-00200).",

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AU - Kwon, Young Guen

AU - Ahn, Youngkeun

AU - Lee, In Se

AU - Won, Moo Ho

N1 - Funding Information: Acknowledgements The authors would like to thank Mr. Seok Han, Mr. Seung Uk Lee and Ms. Hyun Sook Kim for their technical help in this study. This work was supported by the Nano/Bio program of Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (No. M10641450002-06N4145-00200).

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N2 - Doublecortin (DCX), a microtubule-associated protein, specifically expresses in neuronal precursors. This protein has been used as a marker for neuronal precursors and neurogenesis. In the present study, we observed differences in DCX immunoreactivity and its protein levels in the hippocampal dentate gyrus between adult and aged dogs. In the adult dog, DCX immunoreactive cells with well-stained processes were detected in the subgranular zone of the dentate gyrus. Numbers of DCX immunoreactive cells in the dentate gyrus of the aged dog were significantly decreased compared to those in the adult dog. DCX immunoreactive cells in both adult and aged dog did not show NeuN (a marker for mature neurons) immunoreactivity. NeuN immunoreactivity in the aged dog was poor compared to that in the adult dog. DCX protein level in the aged dentate gyrus was decreased by 80% compared to that in the adult dog. These results suggest that the reduction of DCX in the aged hippocampal dentate gyrus may be involved in some neural deficits related to the hippocampus.

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Differences in doublecortin immunoreactivity and protein levels in the hippocampal dentate gyrus between adult and aged dogs

Abstract

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