Diamond-like carbon (DLC) microelectrode for electrochemical ELISA

Jo Il Kim; Adela Bordeanu; Jae Chul Pyun

doi:10.1016/j.bios.2008.08.003

Diamond-like carbon (DLC) microelectrode for electrochemical ELISA

Jo Il Kim, Adela Bordeanu, Jae Chul Pyun

Department of Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

A diamond-like carbon (DLC) microelectrode was applied to commercial ELISA kits for medical diagnosis of HIV (human immunodeficiency virus), HBV (human hepatitis B virus), HCV (human hepatitis C virus). In this work, quantification of oxidized 3,3′,5,5′-tetramethylbenzidine (TMB) was carried out by using a microelectrode made of boron-doped DLC and cyclic voltammetric analysis method without the conventional quenching step which uses sulfuric acid. The microelectrode provided well-known step-shaped graphs, and limit of detection could be improved by clear determination of electrochemical oxidative and reductive peaks. To demonstrate the applicability of DLC microelectrode to conventional ELISA kits, commercial ELISA kits for detection of HIV antigen, HBV antigen and HCV antigen were also tested. These results proved that the applicability of DLC microelectrode to practical detection is feasible.

Original language	English
Pages (from-to)	1394-1398
Number of pages	5
Journal	Biosensors and Bioelectronics
Volume	24
Issue number	5
DOIs	https://doi.org/10.1016/j.bios.2008.08.003
Publication status	Published - 2009 Jan 1

Bibliographical note

Funding Information:
This work was supported by “System IC 2010” project of Ministry of Knowledge Economy, by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (No. R01-2008-000-11767-0), and by Seoul Research and Business Development Program (10816).

All Science Journal Classification (ASJC) codes

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.bios.2008.08.003

Cite this

@article{fe3349cc87134d108487384dfa4eeca4,

title = "Diamond-like carbon (DLC) microelectrode for electrochemical ELISA",

abstract = "A diamond-like carbon (DLC) microelectrode was applied to commercial ELISA kits for medical diagnosis of HIV (human immunodeficiency virus), HBV (human hepatitis B virus), HCV (human hepatitis C virus). In this work, quantification of oxidized 3,3′,5,5′-tetramethylbenzidine (TMB) was carried out by using a microelectrode made of boron-doped DLC and cyclic voltammetric analysis method without the conventional quenching step which uses sulfuric acid. The microelectrode provided well-known step-shaped graphs, and limit of detection could be improved by clear determination of electrochemical oxidative and reductive peaks. To demonstrate the applicability of DLC microelectrode to conventional ELISA kits, commercial ELISA kits for detection of HIV antigen, HBV antigen and HCV antigen were also tested. These results proved that the applicability of DLC microelectrode to practical detection is feasible.",

author = "Kim, {Jo Il} and Adela Bordeanu and Pyun, {Jae Chul}",

note = "Funding Information: This work was supported by “System IC 2010” project of Ministry of Knowledge Economy, by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (No. R01-2008-000-11767-0), and by Seoul Research and Business Development Program (10816).",

year = "2009",

month = jan,

day = "1",

doi = "10.1016/j.bios.2008.08.003",

language = "English",

volume = "24",

pages = "1394--1398",

journal = "Biosensors and Bioelectronics",

issn = "0956-5663",

publisher = "Elsevier Limited",

number = "5",

}

TY - JOUR

T1 - Diamond-like carbon (DLC) microelectrode for electrochemical ELISA

AU - Kim, Jo Il

AU - Bordeanu, Adela

AU - Pyun, Jae Chul

N1 - Funding Information: This work was supported by “System IC 2010” project of Ministry of Knowledge Economy, by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (No. R01-2008-000-11767-0), and by Seoul Research and Business Development Program (10816).

PY - 2009/1/1

Y1 - 2009/1/1

N2 - A diamond-like carbon (DLC) microelectrode was applied to commercial ELISA kits for medical diagnosis of HIV (human immunodeficiency virus), HBV (human hepatitis B virus), HCV (human hepatitis C virus). In this work, quantification of oxidized 3,3′,5,5′-tetramethylbenzidine (TMB) was carried out by using a microelectrode made of boron-doped DLC and cyclic voltammetric analysis method without the conventional quenching step which uses sulfuric acid. The microelectrode provided well-known step-shaped graphs, and limit of detection could be improved by clear determination of electrochemical oxidative and reductive peaks. To demonstrate the applicability of DLC microelectrode to conventional ELISA kits, commercial ELISA kits for detection of HIV antigen, HBV antigen and HCV antigen were also tested. These results proved that the applicability of DLC microelectrode to practical detection is feasible.

AB - A diamond-like carbon (DLC) microelectrode was applied to commercial ELISA kits for medical diagnosis of HIV (human immunodeficiency virus), HBV (human hepatitis B virus), HCV (human hepatitis C virus). In this work, quantification of oxidized 3,3′,5,5′-tetramethylbenzidine (TMB) was carried out by using a microelectrode made of boron-doped DLC and cyclic voltammetric analysis method without the conventional quenching step which uses sulfuric acid. The microelectrode provided well-known step-shaped graphs, and limit of detection could be improved by clear determination of electrochemical oxidative and reductive peaks. To demonstrate the applicability of DLC microelectrode to conventional ELISA kits, commercial ELISA kits for detection of HIV antigen, HBV antigen and HCV antigen were also tested. These results proved that the applicability of DLC microelectrode to practical detection is feasible.

UR - http://www.scopus.com/inward/record.url?scp=58149156560&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58149156560&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2008.08.003

DO - 10.1016/j.bios.2008.08.003

M3 - Article

C2 - 18805687

AN - SCOPUS:58149156560

SN - 0956-5663

VL - 24

SP - 1394

EP - 1398

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 5

ER -

Diamond-like carbon (DLC) microelectrode for electrochemical ELISA

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this