Dexamethasone protects primary cultured hepatocytes from death receptor-mediated apoptosis by upregulation of cFLIP

H. Y. Oh, S. Namkoong, S. J. Lee, E. Por, C. K. Kim, T. R. Billiar, J. A. Han, K. S. Ha, H. T. Chung, Y. G. Kwon, H. Lee, Y. M. Kim

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46 Citations (Scopus)


Dexamethasone (DEX) pretreatment protected hepatocytes from TNF-α plus actinomycin D (ActD)-induced apoptosis by suppressing caspase-8 activation and the mitochondria-dependent apoptosis pathway. DEX treatment upregulated cellular FLICE inhibitory protein (cFLIP) expression, but did not alter the protein levels of Bcl-2, Bcl-xL, Mcl-1, and cIAP as well as Akt activation. The increased cFLIP mRNA level by DEX was inhibited by ActD, indicating that DEX upregulates cFLIP expression at the transcriptional step. DEX also inhibited Jo2-mediated hepatocyte apoptosis by blocking the formation of the death-inducing signaling complex and caspase-8 activation. Specific downregulation of cFLIP expression using siRNA reversed the antiapoptotic effect of DEX by increasing caspase-8 activation. Moreover, DEX administration into mice increased cFLIP expression in the liver and prevented Jo2-induced hepatic injury by inhibiting caspase-8 and -3 activities. Our results indicate that DEX exerts a protective role in death receptor-induced in vitro and in vivo hepatocyte apoptosis by upregulating cFLIP expression.

Original languageEnglish
Pages (from-to)512-523
Number of pages12
JournalCell Death and Differentiation
Issue number3
Publication statusPublished - 2006 Mar

Bibliographical note

Funding Information:
This work was supported by Vascular System Research Center grant from Korea Science and Engineering Foundation.

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology


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