Developmental roles of fuse binding protein 1 (Fubp1) in tooth morphogenesis

Yam Prasad Aryal, Sanjiv Neupane, Tae Young Kim, Eui Seon Lee, Nitin Kumar Pokhrel, Chang Yeol Yeon, Ji Youn Kim, Chang Hyeon An, Seo Young An, Eui Kyun Park, Jung Hong Ha, Jae Kwang Jung, Hitoshi Yamamoto, Sung Won Cho, Sanggyu Lee, Do Yeon Kim, Tae Yub Kwon, Youngkyun Lee, Wern Joo Sohn, Jae Young Kim

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3 Citations (Scopus)


FUSE binding protein 1 (Fubp1), a regulator of the c-Myc transcription factor and a DNA/RNA-binding protein, plays important roles in the regulation of gene transcription and cellular physiology. In this study, to reveal the precise developmental function of Fubp1, we examined the detailed expression pattern and developmental function of Fubp1 during tooth morphogenesis by RT-qPCR, in situ hybridization, and knock-down study using in vitro organ cultivation methods. In embryogenesis, Fubp1 is obviously expressed in the enamel organ and condensed mesenchyme, known to be important for proper tooth formation. Knocking down Fubp1 at E14 for two days, showed the altered expression patterns of tooth development related signalling molecules, including Bmps and Fgf4. In addition, transient knock-down of Fubp1 at E14 revealed changes in the localization patterns of c-Myc and cell proliferation in epithelium and mesenchyme, related with altered tooth morphogenesis. These results also showed the decreased amelogenin and dentin sialophosphoprotein expressions and disrupted enamel rod and interrod formation in one-and three-week renal transplanted teeth respectively. Thus, our results suggested that Fubp1 plays a modulating role during dentinogenesis and amelogenesis by regulating the expression pattern of signalling molecules to achieve the proper structural formation of hard tissue matrices and crown morphogenesis in mice molar development.

Original languageEnglish
Article number8079
Pages (from-to)1-15
Number of pages15
JournalInternational journal of molecular sciences
Issue number21
Publication statusPublished - 2020 Nov 1

Bibliographical note

Funding Information:
Funding: This study was supported by the National Research Foundation of Korea (grant NRF-2018R1A2A3075600, 2017R1A5A2015391 and 2015R1A2A2A01008180) funded by the Ministry of Education, Science and Technology, Republic of Korea.

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry


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