Developmental regulations of Perp in mice molar morphogenesis

Sanjiv Neupane, Wern Joo Sohn, Girdhari Rijal, Ye Ji Lee, Sanggyu Lee, Hitoshi Yamamoto, Chang Hyeon An, Sung Won Cho, Youngkyun Lee, Hong In Shin, Tae Yub Kwon, Jae Young Kim

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Teraspanin transmembrane protein, Perp (P53 apoptosis effector related to PMP22), which is found in the plasma membrane as a component of the desmosome, is reported to be involved in the morphogenesis of the epithelium and the enamel formation of the incisor. However, its expression pattern and signaling regulation during molar development have not been elucidated in detail. We have examined the precise expression patterns of Perp in developing lower molars and employed the knock-down of Perp by antisense oligodeoxynucleotide treatment during in vitro organ cultivation at embryonic day 13 to define the precise developmental function of Perp. Perp was expressed mainly in the dental lamina and stellate reticulum regions at the bud and cap stages. After Perp knock-down, the tooth germ showed disruption of the dental lamina and stellate reticulum with altered apoptosis and proliferation. The changed expression levels of related signaling molecules from the enamel knot and desmosome were evaluated by real-time quantitative polymerase chain reaction. A renal capsule transplantation method was employed to examine the effects of Perp knock-down on molar crown development. Ultrastructural observations revealed that enamel was deposited more densely in an irregular pattern in the cusp region, and that dentin was hypo-mineralized after Perp knock-down at the cap stage. Thus, Perp might play important roles in the formation and integration of stellate reticulum, dental lamina structure and enamel formation through signaling interactions with the enamel knot and desmosome-related signaling molecules at the cap stage of lower molar development.

Original languageEnglish
Pages (from-to)109-121
Number of pages13
JournalCell and Tissue Research
Issue number1
Publication statusPublished - 2014 Oct 7

Bibliographical note

Publisher Copyright:
© 2014, Springer-Verlag Berlin Heidelberg.

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology


Dive into the research topics of 'Developmental regulations of Perp in mice molar morphogenesis'. Together they form a unique fingerprint.

Cite this