TY - GEN
T1 - Development of phenol detecting biosensor using PEG hydrogel microparticles
AU - Jang, Eunji
AU - Park, Saemi
AU - Lee, Hyun Jong
AU - Murthy, Keshava
AU - Koh, Won Gun
PY - 2010
Y1 - 2010
N2 - In this study, a simple and sensitive detection method for phenols were investigated using poly(ethylene glycol) (PEG) hydrogel microarray entrapping quantum dot (QD) and tyrosinase enzyme. Hydrogel microarrays were fabricated by using photo-patterning methods, and size of resultant hydrogel micropattern was ranged from 10 to 300 μm. The enzymatic reaction used for the detection of phenol is the oxidation of phenolic compounds via tyrosinase. In such reaction, phenol is oxidized to o-quinone which is a classic electron accepting chemical that can quench the fluorescence of QDs. Accuracy of the system was verified by measurement of photoluminescence (PL) quenching in free solution state with different concentrations of phenol. The PL quenching of QDs was investigated by varying the molecular weight of PEG, and concentration of QDs and/or enzymes.
AB - In this study, a simple and sensitive detection method for phenols were investigated using poly(ethylene glycol) (PEG) hydrogel microarray entrapping quantum dot (QD) and tyrosinase enzyme. Hydrogel microarrays were fabricated by using photo-patterning methods, and size of resultant hydrogel micropattern was ranged from 10 to 300 μm. The enzymatic reaction used for the detection of phenol is the oxidation of phenolic compounds via tyrosinase. In such reaction, phenol is oxidized to o-quinone which is a classic electron accepting chemical that can quench the fluorescence of QDs. Accuracy of the system was verified by measurement of photoluminescence (PL) quenching in free solution state with different concentrations of phenol. The PL quenching of QDs was investigated by varying the molecular weight of PEG, and concentration of QDs and/or enzymes.
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U2 - 10.1109/INEC.2010.5425132
DO - 10.1109/INEC.2010.5425132
M3 - Conference contribution
AN - SCOPUS:77951664470
SN - 9781424435449
T3 - INEC 2010 - 2010 3rd International Nanoelectronics Conference, Proceedings
SP - 909
EP - 910
BT - INEC 2010 - 2010 3rd International Nanoelectronics Conference, Proceedings
T2 - 2010 3rd International Nanoelectronics Conference, INEC 2010
Y2 - 3 January 2010 through 8 January 2010
ER -