Development of a sensitive SPR biosensor for C-reactive protein (CRP) using plasma-treated parylene-N film

Yong Hwan Choi, Hyuk Ko, Ga Yeon Lee, Seo Yoon Chang, Young Wook Chang, Min Jung Kang, Jae Chul Pyun

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


Parylene-N film has high protein immobilization property after plasma-treatment. In this work, the optimum conditions for plasma-treatment of parylene-N films were investigated to develop a highly sensitive SPR biosensor. The effects of the plasma power and plasma-treatment time on the surface of parylene-N films were analyzed by measuring film contact angle and roughness from atomic force microscopy images. The plasma power was applied at a range of 0-100 W for different treatment times. The influence of plasma-treatment was also analyzed using the depth profiles from X-ray photospectroscopy. From this analysis, chemical functional groups (those related to oxygen) were introduced and were estimated to penetrate up to 4 nm and 8 nm from the top surface of the parylene-N film due to treatment with plasma powers of 50 W and 100 W, respectively, for 1 min. Based on the optimum plasma-treatment conditions for the plasma power and the plasma-treatment time, an SPR biosensor was developed for the detection of the human C-reactive protein.

Original languageEnglish
Pages (from-to)133-138
Number of pages6
JournalSensors and Actuators, B: Chemical
Issue numberPart A
Publication statusPublished - 2015 Feb

Bibliographical note

Funding Information:
This research was supported by the National Research Foundation of Korea ( 2013-056267 , 2013-056331 and 2013R1A1A2065723 ). This work was also supported by the Creative Fusion Research Program through the Creative Allied Project funded by Korea Research Council of Fundamental Science and Technology ( CAP-12-1 ).

Publisher Copyright:
© 2014 Elsevier B.V. All rights reserved.

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry


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