Abstract
Parylene-N film has high protein immobilization property after plasma-treatment. In this work, the optimum conditions for plasma-treatment of parylene-N films were investigated to develop a highly sensitive SPR biosensor. The effects of the plasma power and plasma-treatment time on the surface of parylene-N films were analyzed by measuring film contact angle and roughness from atomic force microscopy images. The plasma power was applied at a range of 0-100 W for different treatment times. The influence of plasma-treatment was also analyzed using the depth profiles from X-ray photospectroscopy. From this analysis, chemical functional groups (those related to oxygen) were introduced and were estimated to penetrate up to 4 nm and 8 nm from the top surface of the parylene-N film due to treatment with plasma powers of 50 W and 100 W, respectively, for 1 min. Based on the optimum plasma-treatment conditions for the plasma power and the plasma-treatment time, an SPR biosensor was developed for the detection of the human C-reactive protein.
| Original language | English |
|---|---|
| Pages (from-to) | 133-138 |
| Number of pages | 6 |
| Journal | Sensors and Actuators, B: Chemical |
| Volume | 207 |
| Issue number | Part A |
| DOIs | |
| Publication status | Published - 2015 Feb |
Bibliographical note
Funding Information:This research was supported by the National Research Foundation of Korea ( 2013-056267 , 2013-056331 and 2013R1A1A2065723 ). This work was also supported by the Creative Fusion Research Program through the Creative Allied Project funded by Korea Research Council of Fundamental Science and Technology ( CAP-12-1 ).
Publisher Copyright:
© 2014 Elsevier B.V. All rights reserved.
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Instrumentation
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry