Development of a polymerase chain reaction test to confirm Mycobacterium avium subsp. paratuberculosis in culture

Sung Jae Shin; Yung Fu Chang; Cathy Huang; Jiaqian Zhu; Lester Huang; Han Sang Yoo; Kwang Soon Shin; Susan Stehman; Sang J. Shin; Alfonso Torres

doi:10.1177/104063870401600204

Development of a polymerase chain reaction test to confirm Mycobacterium avium subsp. paratuberculosis in culture

Sung Jae Shin, Yung Fu Chang, Cathy Huang, Jiaqian Zhu, Lester Huang, Han Sang Yoo, Kwang Soon Shin, Susan Stehman, Sang J. Shin, Alfonso Torres

Department of Microbiology

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

A polymerase chain reaction (PCR) assay for confirmation of Mycobacterium avium subsp. paratuberculosis was developed using the primer set derived from ISMav2. The PCR product was 494 base pairs (bp) and could be digested with ClaI, which produced 311- and 183-bp fragments. No amplification of 494-bp DNA fragment was detected from DNA of other Mycobacterium spp., including Mycobacterium avium complex, other bacteria, including Escherichia coli, Pseudomonas aeruginosa, Actinobacillus pleuropneumoniae, Leptospira interrogans serovar pomona, Corynebacterium pseudotuberculosis, Salmonella typhimurium, Borrelia burgdorferi, and Staphylococcus aureus, and the Scedosporium sp. This PCR assay could detect 5-8 genome equivalents.

Original language	English
Pages (from-to)	116-120
Number of pages	5
Journal	Journal of Veterinary Diagnostic Investigation
Volume	16
Issue number	2
DOIs	https://doi.org/10.1177/104063870401600204
Publication status	Published - 2004 Mar

All Science Journal Classification (ASJC) codes

veterinary(all)

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10.1177/104063870401600204

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title = "Development of a polymerase chain reaction test to confirm Mycobacterium avium subsp. paratuberculosis in culture",

abstract = "A polymerase chain reaction (PCR) assay for confirmation of Mycobacterium avium subsp. paratuberculosis was developed using the primer set derived from ISMav2. The PCR product was 494 base pairs (bp) and could be digested with ClaI, which produced 311- and 183-bp fragments. No amplification of 494-bp DNA fragment was detected from DNA of other Mycobacterium spp., including Mycobacterium avium complex, other bacteria, including Escherichia coli, Pseudomonas aeruginosa, Actinobacillus pleuropneumoniae, Leptospira interrogans serovar pomona, Corynebacterium pseudotuberculosis, Salmonella typhimurium, Borrelia burgdorferi, and Staphylococcus aureus, and the Scedosporium sp. This PCR assay could detect 5-8 genome equivalents.",

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T1 - Development of a polymerase chain reaction test to confirm Mycobacterium avium subsp. paratuberculosis in culture

AU - Shin, Sung Jae

AU - Chang, Yung Fu

AU - Huang, Cathy

AU - Zhu, Jiaqian

AU - Huang, Lester

AU - Yoo, Han Sang

AU - Shin, Kwang Soon

AU - Stehman, Susan

AU - Shin, Sang J.

AU - Torres, Alfonso

PY - 2004/3

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Development of a polymerase chain reaction test to confirm Mycobacterium avium subsp. paratuberculosis in culture

Abstract

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