Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis

J. Nylk; M. V.G. Kristensen; M. Mazilu; A. K. Thayil; C. A. Mitchell; E. C. Campbell; S. J. Powis; F. J. Gunn-Moore; K. Dholakia

doi:10.1364/BOE.6.001512

Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis

J. Nylk, M. V.G. Kristensen, M. Mazilu, A. K. Thayil, C. A. Mitchell, E. C. Campbell, S. J. Powis, F. J. Gunn-Moore, K. Dholakia

Department of Physics

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200mm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.

Original language	English
Article number	A036
Pages (from-to)	1512-1519
Number of pages	8
Journal	Biomedical Optics Express
Volume	6
Issue number	4
DOIs	https://doi.org/10.1364/BOE.6.001512
Publication status	Published - 2015

Bibliographical note

Publisher Copyright:
© 2015 Optical Society of America.

All Science Journal Classification (ASJC) codes

Biotechnology
Atomic and Molecular Physics, and Optics

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10.1364/BOE.6.001512

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abstract = "We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200mm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.",

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AU - Nylk, J.

AU - Kristensen, M. V.G.

AU - Mazilu, M.

AU - Thayil, A. K.

AU - Mitchell, C. A.

AU - Campbell, E. C.

AU - Powis, S. J.

AU - Gunn-Moore, F. J.

AU - Dholakia, K.

PY - 2015

Y1 - 2015

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AB - We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200mm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.

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Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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