Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy

Vincent O. Baron; Mingzhou Chen; Simon O. Clark; Ann Williams; Kishan Dholakia; Stephen H. Gillespie

doi:10.1007/978-1-4939-7638-6_4

Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy

Vincent O. Baron, Mingzhou Chen, Simon O. Clark, Ann Williams, Kishan Dholakia, Stephen H. Gillespie

Department of Physics

Research output: Chapter in Book/Report/Conference proceeding › Chapter

10 Citations (Scopus)

Abstract

Raman spectroscopy is a non-destructive and label-free technique. Wavelength modulated Raman (WMR) spectroscopy was applied to investigate Mycobacterium tuberculosis cell state, lipid rich (LR) and lipid poor (LP). Compared to LP cells, LR cells can be up to 40 times more resistant to key antibiotic regimens. Using this methodology single lipid rich (LR) from lipid poor (LP) bacteria can be differentiated with both high sensitivity and specificity. It can also be used to investigate experimentally infected frozen tissue sections where both cell types can be differentiated. This methodology could be utilized to study the phenotype of mycobacterial cells in other tissues.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	41-50
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-4939-7638-6_4
Publication status	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1736
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© 2018, Springer Science+Business Media, LLC.

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-7638-6_4

Cite this

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abstract = "Raman spectroscopy is a non-destructive and label-free technique. Wavelength modulated Raman (WMR) spectroscopy was applied to investigate Mycobacterium tuberculosis cell state, lipid rich (LR) and lipid poor (LP). Compared to LP cells, LR cells can be up to 40 times more resistant to key antibiotic regimens. Using this methodology single lipid rich (LR) from lipid poor (LP) bacteria can be differentiated with both high sensitivity and specificity. It can also be used to investigate experimentally infected frozen tissue sections where both cell types can be differentiated. This methodology could be utilized to study the phenotype of mycobacterial cells in other tissues.",

keywords = "Lipids, Mycobacteria, Phenotypic resistance, Raman spectroscopy",

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AU - Chen, Mingzhou

AU - Clark, Simon O.

AU - Williams, Ann

AU - Dholakia, Kishan

AU - Gillespie, Stephen H.

PY - 2018

Y1 - 2018

N2 - Raman spectroscopy is a non-destructive and label-free technique. Wavelength modulated Raman (WMR) spectroscopy was applied to investigate Mycobacterium tuberculosis cell state, lipid rich (LR) and lipid poor (LP). Compared to LP cells, LR cells can be up to 40 times more resistant to key antibiotic regimens. Using this methodology single lipid rich (LR) from lipid poor (LP) bacteria can be differentiated with both high sensitivity and specificity. It can also be used to investigate experimentally infected frozen tissue sections where both cell types can be differentiated. This methodology could be utilized to study the phenotype of mycobacterial cells in other tissues.

AB - Raman spectroscopy is a non-destructive and label-free technique. Wavelength modulated Raman (WMR) spectroscopy was applied to investigate Mycobacterium tuberculosis cell state, lipid rich (LR) and lipid poor (LP). Compared to LP cells, LR cells can be up to 40 times more resistant to key antibiotic regimens. Using this methodology single lipid rich (LR) from lipid poor (LP) bacteria can be differentiated with both high sensitivity and specificity. It can also be used to investigate experimentally infected frozen tissue sections where both cell types can be differentiated. This methodology could be utilized to study the phenotype of mycobacterial cells in other tissues.

KW - Lipids

KW - Mycobacteria

KW - Phenotypic resistance

KW - Raman spectroscopy

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Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy

Abstract

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Bibliographical note

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UN SDGs

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