Design of biocompatible block catiomers directed to effective transfection with minimal toxicity

Nobuhiro Nisihiyama; Keiji Itaka; Shigeto Fukushima; Woo Dong Jang; Naoki Kanayama; Kanjiro Miyata; Shunsaku Asano; Ung Li Chung; Yuichi Yamasaki; Kazunori Kataoka

Design of biocompatible block catiomers directed to effective transfection with minimal toxicity

Nobuhiro Nisihiyama, Keiji Itaka, Shigeto Fukushima, Woo Dong Jang, Naoki Kanayama, Kanjiro Miyata, Shunsaku Asano, Ung Li Chung, Yuichi Yamasaki, Kazunori Kataoka

Department of Chemistry

Research output: Contribution to conference › Paper › peer-review

Abstract

To develop biocompatible non-viral gene carriers, poly(ethylene glycol)(PEG)-based block catiomers carrying the ethylene diamine moiety (PEG-P[Asp(DET)]) in the side chain were prepared through the aminolysis reaction of PEG-poly(ß-benzyl L-aspartate) (PEG-PBLA) with 50 eq. of diethylenetriamine (DET) in DMF. PEG-P[Asp(DET)] formed the polyplex micelles with the size of ∼100 nm and almost neutral ζ-potential, which are characterized by the structure of the packaged DNA surrounded by the PEG outer shell. Biological studies have revealed that the polyplex micelles show the efficient transfection and remarkably low cytotoxicity toward primary cells. Thus, the PEG-P[Asp(DET)] system might be a promising vector for in vivo topical gene therapy.

Original language	English
Pages	5108-5109
Number of pages	2
Publication status	Published - 2005
Event	54th SPSJ Symposium on Macromolecules - Yamagata, Japan Duration: 2005 Sept 20 → 2005 Sept 22

Other

Other	54th SPSJ Symposium on Macromolecules
Country/Territory	Japan
City	Yamagata
Period	05/9/20 → 05/9/22

All Science Journal Classification (ASJC) codes

Engineering(all)

Cite this

@conference{2d4a9123d0644dc69e5209e2a150b029,

title = "Design of biocompatible block catiomers directed to effective transfection with minimal toxicity",

abstract = "To develop biocompatible non-viral gene carriers, poly(ethylene glycol)(PEG)-based block catiomers carrying the ethylene diamine moiety (PEG-P[Asp(DET)]) in the side chain were prepared through the aminolysis reaction of PEG-poly({\ss}-benzyl L-aspartate) (PEG-PBLA) with 50 eq. of diethylenetriamine (DET) in DMF. PEG-P[Asp(DET)] formed the polyplex micelles with the size of ∼100 nm and almost neutral ζ-potential, which are characterized by the structure of the packaged DNA surrounded by the PEG outer shell. Biological studies have revealed that the polyplex micelles show the efficient transfection and remarkably low cytotoxicity toward primary cells. Thus, the PEG-P[Asp(DET)] system might be a promising vector for in vivo topical gene therapy.",

author = "Nobuhiro Nisihiyama and Keiji Itaka and Shigeto Fukushima and Jang, {Woo Dong} and Naoki Kanayama and Kanjiro Miyata and Shunsaku Asano and Chung, {Ung Li} and Yuichi Yamasaki and Kazunori Kataoka",

year = "2005",

language = "English",

pages = "5108--5109",

note = "54th SPSJ Symposium on Macromolecules ; Conference date: 20-09-2005 Through 22-09-2005",

}

TY - CONF

T1 - Design of biocompatible block catiomers directed to effective transfection with minimal toxicity

AU - Nisihiyama, Nobuhiro

AU - Itaka, Keiji

AU - Fukushima, Shigeto

AU - Jang, Woo Dong

AU - Kanayama, Naoki

AU - Miyata, Kanjiro

AU - Asano, Shunsaku

AU - Chung, Ung Li

AU - Yamasaki, Yuichi

AU - Kataoka, Kazunori

PY - 2005

Y1 - 2005

N2 - To develop biocompatible non-viral gene carriers, poly(ethylene glycol)(PEG)-based block catiomers carrying the ethylene diamine moiety (PEG-P[Asp(DET)]) in the side chain were prepared through the aminolysis reaction of PEG-poly(ß-benzyl L-aspartate) (PEG-PBLA) with 50 eq. of diethylenetriamine (DET) in DMF. PEG-P[Asp(DET)] formed the polyplex micelles with the size of ∼100 nm and almost neutral ζ-potential, which are characterized by the structure of the packaged DNA surrounded by the PEG outer shell. Biological studies have revealed that the polyplex micelles show the efficient transfection and remarkably low cytotoxicity toward primary cells. Thus, the PEG-P[Asp(DET)] system might be a promising vector for in vivo topical gene therapy.

AB - To develop biocompatible non-viral gene carriers, poly(ethylene glycol)(PEG)-based block catiomers carrying the ethylene diamine moiety (PEG-P[Asp(DET)]) in the side chain were prepared through the aminolysis reaction of PEG-poly(ß-benzyl L-aspartate) (PEG-PBLA) with 50 eq. of diethylenetriamine (DET) in DMF. PEG-P[Asp(DET)] formed the polyplex micelles with the size of ∼100 nm and almost neutral ζ-potential, which are characterized by the structure of the packaged DNA surrounded by the PEG outer shell. Biological studies have revealed that the polyplex micelles show the efficient transfection and remarkably low cytotoxicity toward primary cells. Thus, the PEG-P[Asp(DET)] system might be a promising vector for in vivo topical gene therapy.

UR - http://www.scopus.com/inward/record.url?scp=33645566948&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645566948&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33645566948

SP - 5108

EP - 5109

T2 - 54th SPSJ Symposium on Macromolecules

Y2 - 20 September 2005 through 22 September 2005

ER -

Design of biocompatible block catiomers directed to effective transfection with minimal toxicity

Abstract

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All Science Journal Classification (ASJC) codes

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